Figure 1. NDP-a-MSH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/MC4 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist NDP-a-MSH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NDP-a-MSH (Mean ± SD, n = 2). The EC50 of NDP-a-MSH on this cell was 0.30 μM.
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/MC4/Gα15 Stable Cell Line
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