Catalog Products » Stable Cell Lines » Human Recombinant MC4 Melanocortin Receptor Stable Cell Line
CHO-K1/MC4/Gα15 Stable Cell Line

Figure 1. NDP-a-MSH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/MC4 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist NDP-a-MSH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NDP-a-MSH (Mean ± SD, n = 2). The EC50 of NDP-a-MSH on this cell was 0.30 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/MC4/Gα15 Stable Cell Line

The melanocortin family consists of five melanocortin G-Protein-coupled receptors (designated MC1-5R). The melanocortins, α-, β- and γ-melanocyte-stimulating hormones (MSHs), are peptides derived from a precursor protein POMC, and play important roles in energy balance, reproductive function, pigmentation and inflammation. MC4 receptors with high affinities for α-MSH and ACTH are expressed almost exclusively in the CNS and appear to play a prominent role in energy homeostasis.
M00272
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Description

The melanocortin family consists of five melanocortin G-Protein-coupled receptors (designated MC1-5R). The melanocortins, α-, β- and γ-melanocyte-stimulating hormones (MSHs), are peptides derived from a precursor protein POMC, and play important roles in energy balance, reproductive function, pigmentation and inflammation. MC4 receptors with high affinities for α-MSH and ACTH are expressed almost exclusively in the CNS and appear to play a prominent role in energy homeostasis.

Synonyms

MC4 receptor, MC4R, melanocortin receptor 4, MC4-R

Overview
Applications Functional assay for MC4 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/MC4/Gα15 Stable Cell Line
  • CHO-K1/MC4/Gα15 Stable Cell Line

    Figure 1. NDP-a-MSH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/MC4 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist NDP-a-MSH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NDP-a-MSH (Mean ± SD, n = 2). The EC50 of NDP-a-MSH on this cell was 0.30 μM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom


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