Catalog Products » Stable Cell Lines » Human Recombinant SST5 Somatostatin Receptor Stable Cell Line

CHO-K1/SST5/Gα15 Stable Cell Line

Somatostatin receptors (SSTRs), a family of seven transmembrane (TM) domain G-protein-coupled receptors having five distinct subtypes (termed SSTR1–5), are activated by somatostatin secreted from the nerve and endocrine cells. SSTRs are widely expressed in many tissues, frequently as multiple subtypes that coexist in the same cell. With expressions in a tissue-specific manner, SSTRs are involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. The five receptors share common signaling pathways such as the inhibition of adenylyl cyclase, activation of phosphotyrosine phosphatase (PTP), and modulation of mitogen-activated protein kinase (MAPK) through G-protein-dependent mechanisms. Aberrant expression of somatostatin receptors is known to be involved in a large number of human tumors. The human medullary thyroid carcinoma cell line TT expresses all SSTR subtypes. SSTR5 induces cell cycle arrest via PTP-dependent modulation of MAPK, which is associated with the induction of the retinoblastoma tumor suppressor protein and p21. In addition, SSTR 5 displays acute desensitization of adenylyl cyclase coupling and undergoes rapid agonist-dependent endocytosis.
M00286
$8,500.00

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Description

Somatostatin receptors (SSTRs), a family of seven transmembrane (TM) domain G-protein-coupled receptors having five distinct subtypes (termed SSTR1–5), are activated by somatostatin secreted from the nerve and endocrine cells. SSTRs are widely expressed in many tissues, frequently as multiple subtypes that coexist in the same cell. With expressions in a tissue-specific manner, SSTRs are involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. The five receptors share common signaling pathways such as the inhibition of adenylyl cyclase, activation of phosphotyrosine phosphatase (PTP), and modulation of mitogen-activated protein kinase (MAPK) through G-protein-dependent mechanisms. Aberrant expression of somatostatin receptors is known to be involved in a large number of human tumors. The human medullary thyroid carcinoma cell line TT expresses all SSTR subtypes. SSTR5 induces cell cycle arrest via PTP-dependent modulation of MAPK, which is associated with the induction of the retinoblastoma tumor suppressor protein and p21. In addition, SSTR 5 displays acute desensitization of adenylyl cyclase coupling and undergoes rapid agonist-dependent endocytosis.

Synonyms

sst5 receptor, SSTR5, SRIF1, SRIF1B, SOMATO, Smstr5, SS-5-R, SS5-R, SS5R, Somatostatin receptor subtype 5, somatostatin receptor type 5, sst5

Overview
Applications Functional assay for SST5 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)


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