Catalog Products » Stable Cell Lines » Human Recombinant Parathyroid Hormone Receptor 1 Stable Cell Line
CHO-K1/PTH1/Gα15 Stable Cell Line

Figure 1. PTH (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTH/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PTH (1-34). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of PTH (1-34) (Mean ± SD, n = 2). The EC50 of PTH (1-34) on this cell was 0.26 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/PTH1/Gα15 Stable Cell Line

Parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor (PTHR1) is a G protein coupled receptor which mediates the actions of both amino-terminal PTH and PTHrP fragments. The most abundant expression of PTHR1 is found in renal tubular cells and in osteoblasts, where the PTH/PTHrP receptor mediates the endocrine actions of PTH, and in prehypertrophic chondrocytes of the metaphyseal growth plate, where it mediates the autocrine/paracrine actions of PTHrP. Intact PTH (PTH 1-84) is compounded by a peptide of 84 amino acids (AA), the amino-terminal sequence, constituted by the first 34 AA (N-terminal structure), is necessary for its action.
M00315
$8,500.00

Ask us a question
Description

Parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor (PTHR1) is a G protein coupled receptor which mediates the actions of both amino-terminal PTH and PTHrP fragments. The most abundant expression of PTHR1 is found in renal tubular cells and in osteoblasts, where the PTH/PTHrP receptor mediates the endocrine actions of PTH, and in prehypertrophic chondrocytes of the metaphyseal growth plate, where it mediates the autocrine/paracrine actions of PTHrP. Intact PTH (PTH 1-84) is compounded by a peptide of 84 amino acids (AA), the amino-terminal sequence, constituted by the first 34 AA (N-terminal structure), is necessary for its action.

Synonyms

PTH1 receptor, PPR, PTH/PTHrP receptor, PTH/PTHrP, PTHR, PTHR1, parathyroid hormone receptor 1, PTHrel, Pth1r, PTH/PTHr receptor, PTH/PTHrP type I receptor, Parathyroid hormone/parathyroid hormone-related peptide receptor, parathyroid hormone 1 receptor, parathyroid hormone receptor, PTH-related peptide receptor

Overview
Applications Functional assay for PTHR1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 400 μg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/PTH1/Gα15 Stable Cell Line
  • CHO-K1/PTH1/Gα15 Stable Cell Line

    Figure 1. PTH (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTH/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PTH (1-34). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of PTH (1-34) (Mean ± SD, n = 2). The EC50 of PTH (1-34) on this cell was 0.26 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


Do you like the current new website?

Hate

Dislike

Neutral

Like

Love

*