Catalog Products » Stable Cell Lines » Human Recombinant OX2 Orexin Receptor Stable Cell Line
CHO-K1/OX2 Stable Cell Line

Figure 1. Orexin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OX2 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist orexin A. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of orexin A (Mean ± SD, n = 2). The EC50 of orexin A on this cell was 1.3 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/OX2 Stable Cell Line

Hypocretin (orexin) receptor 2, also known as OX2, is a human protein encoded by the HCRTR2 gene. Orexin A and orexin B are neuropeptides originally identified as endogenous ligands for OX2. Orexin neuropeptides are produced by a small group of neurons in the lateral hypothalamic and perifornical areas, a region that is classically implicated with the control of mammalian feeding behavior. Orexin neurons that project throughout the central nervous system to nuclei are known to be important in the control of feeding, sleep-wakefulness, neuroendocrine homeostasis, and autonomic regulation.
M00316
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Description

Hypocretin (orexin) receptor 2, also known as OX2, is a human protein encoded by the HCRTR2 gene. Orexin A and orexin B are neuropeptides originally identified as endogenous ligands for OX2. Orexin neuropeptides are produced by a small group of neurons in the lateral hypothalamic and perifornical areas, a region that is classically implicated with the control of mammalian feeding behavior. Orexin neurons that project throughout the central nervous system to nuclei are known to be important in the control of feeding, sleep-wakefulness, neuroendocrine homeostasis, and autonomic regulation.

Synonyms

OX2 receptor, hypocretin receptor type 2, OX2R, hypocretin (orexin) receptor 2, hypocretin receptor 2, orexin receptor type 2, ox-2-R, mOXR2, mOX2aR, mOX2bR

Overview
Applications Functional assay for OX2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/OX2 Stable Cell Line
  • CHO-K1/OX2 Stable Cell Line

    Figure 1. Orexin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OX2 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist orexin A. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of orexin A (Mean ± SD, n = 2). The EC50 of orexin A on this cell was 1.3 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


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