
Figure 1: ATP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y2 cells. The cells were loaded with Calcium-4 prior to stimulation with a P2Y2 receptor agonist, ATP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ATP (Mean ± SEM, n = 3). The EC50 of ATP on P2Y2 in 1321N1 cells was 32.38 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.
1321N1/P2Y2 Stable Cell Line
M00318 | |
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$8,500.00 | |
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