Catalog Products » Stable Cell Lines » Human Recombinant δ-Opioid Receptor OPRD1 Stable Cell Line
CHO-K1/OPRD1/Gα15 Stable Cell Line

Figure 1. SNC80-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRD1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist SNC80. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of SNC80 (Mean ± SD, n = 2). The EC50 of SNC80 on this cell was 0.75 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/OPRD1/Gα15 Stable Cell Line

Figure 2. 5 μg of membranes prepared from CHO-K1 cells stably expressing OPRD1 receptors were incubated with indicated concentrations of [3H]Naltrindole in the absence (total binding) or presence of 1000-fold excess unlabeled Naloxone (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

CHO-K1/OPRD1/Gα15 Stable Cell Line

Figure 3. 5 μg of membranes prepared from CHO-K1 cells stably expressing OPRD1 receptors were incubated with indicated concentrations of Naloxone in the presence of 0.4 nM [3H]Naltrindole. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

CHO-K1/OPRD1/Gα15 Stable Cell Line

Opioid receptor family includes three classic receptors, μ, δ, and κ, also known as OP1, OP2 and OP3, respectively. The receptors are Gi/o-coupled GPCRs which will reduce intracellular cAMP levels after activation. δ-opioid receptor modulates many kinase cascades including ERKs, Akts, JNKs, STAT3, P38 involving Src, Ras, Rac, Raf-1, Cdc42, RTKs. In addition, δ-opioid receptor has also been proposed to interact with μ receptors. The observed pharmacological cross-talk may partially arise from agonist cross-reactivity.
M00323
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Description

Opioid receptor family includes three classic receptors, μ, δ, and κ, also known as OP1, OP2 and OP3, respectively. The receptors are Gi/o-coupled GPCRs which will reduce intracellular cAMP levels after activation. δ-opioid receptor modulates many kinase cascades including ERKs, Akts, JNKs, STAT3, P38 involving Src, Ras, Rac, Raf-1, Cdc42, RTKs. In addition, δ-opioid receptor has also been proposed to interact with μ receptors. The observed pharmacological cross-talk may partially arise from agonist cross-reactivity.

Synonyms

DOR, OP1, DOP, Delta opioid receptor, Opioid receptor delta 1, delta-type opioid receptor, opioid receptor A, Nbor, mDOR, DOP-r

Overview
Applications Functional assay for OPRD1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/OPRD1/Gα15 Stable Cell Line
  • CHO-K1/OPRD1/Gα15 Stable Cell Line

    Figure 1. SNC80-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRD1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist SNC80. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of SNC80 (Mean ± SD, n = 2). The EC50 of SNC80 on this cell was 0.75 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom

  • CHO-K1/OPRD1/Gα15 Stable Cell Line
  • CHO-K1/OPRD1/Gα15 Stable Cell Line

    Figure 2. 5 μg of membranes prepared from CHO-K1 cells stably expressing OPRD1 receptors were incubated with indicated concentrations of [3H]Naltrindole in the absence (total binding) or presence of 1000-fold excess unlabeled Naloxone (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

  • CHO-K1/OPRD1/Gα15 Stable Cell Line
  • CHO-K1/OPRD1/Gα15 Stable Cell Line

    Figure 3. 5 μg of membranes prepared from CHO-K1 cells stably expressing OPRD1 receptors were incubated with indicated concentrations of Naloxone in the presence of 0.4 nM [3H]Naltrindole. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.


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