Catalog Products » Stable Cell Lines » Human Recombinant Complement Component 5A Receptor 1 Stable Cell Line
CHO-K1/C5a/Gα15 Stable Cell Line

Figure 1. C5a (65-74)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/C5a/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist C5a (65-74). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of C5a (65-74) (Mean ± SD, n = 2). The EC50 of C5a (65-74) on this cell was 4.2 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/C5a/Gα15 Stable Cell Line

The complement component 5a receptor 1 (C5a) is Gq/11 and Gi/o-coupled GPCRs. C5a is a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a receptor plays an important role in mediating acute kidney allograft rejection.
M00327
$8,500.00

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Description

The complement component 5a receptor 1 (C5a) is Gq/11 and Gi/o-coupled GPCRs. C5a is a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a receptor plays an important role in mediating acute kidney allograft rejection.

Synonyms

C5a1 receptor, C5aR1, CD88, C5R1, C5A, C5AR, complement component 5 receptor 1 (C5a ligand), C5a anaphylatoxin chemotactic receptor, C5a-R, complement C5a receptor, complement component 5, receptor 1, complement component 5a receptor 1, D7Msu1

Overview
Applications Functional assay for C5a receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 400 μg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/C5a/Gα15 Stable Cell Line
  • CHO-K1/C5a/Gα15 Stable Cell Line

    Figure 1. C5a (65-74)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/C5a/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist C5a (65-74). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of C5a (65-74) (Mean ± SD, n = 2). The EC50 of C5a (65-74) on this cell was 4.2 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


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