Catalog Products » Stable Cell Lines » Human Recombinant 5-HT1A Serotonin Receptor Stable Cell Line
CHO-K1/5-HT1A/Gα15 Stable Cell Line

Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT1A/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist 5-HT. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on this cell was 17.7 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/5-HT1A/Gα15 Stable Cell Line

Figure 2 10 μg of membranes prepared from CHO-K1 cells stably expressing 5-HT1A receptors were incubated with indicated concentrations of [3H]5-HT in the absence (total binding) or presence of 1000-fold access unlabeled Serotonin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

CHO-K1/5-HT1A/Gα15 Stable Cell Line

Figure 3 10 μg of membranes prepared from CHO-K1 cells stably expressing 5-HT1A receptors were incubated with indicated concentrations of 5-HT in the presence of 2 nM [3H]5-HT. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

CHO-K1/5-HT1A/Gα15 Stable Cell Line

The 5-Hydroxytryptamine receptor 1A (5-HT1A) is Gi-coupled GPCRs expressed in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus, with lesser amounts in the basal ganglia and thalamus. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and schizophrenia.
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Description

The 5-Hydroxytryptamine receptor 1A (5-HT1A) is Gi-coupled GPCRs expressed in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus, with lesser amounts in the basal ganglia and thalamus. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and schizophrenia.

Synonyms

5-HT1A receptor, ADRB2RL1, ADRBRL1, 5-HT1A, 5-hydroxytryptamine (serotonin) receptor 1A, 5HT1A, RAT5HT1A, 5-HT-1A, 5-hydroxytryptamine receptor 1A, serotonin receptor 1A, 5-HT1A receptor, Gpcr18

Overview
Applications Functional assay for 5-HT1A receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12, 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)

Examples
  • CHO-K1/5-HT1A/Gα15 Stable Cell Line
  • CHO-K1/5-HT1A/Gα15 Stable Cell Line

    Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT1A/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist 5-HT. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on this cell was 17.7 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

  • CHO-K1/5-HT1A/Gα15 Stable Cell Line
  • CHO-K1/5-HT1A/Gα15 Stable Cell Line

    Figure 2 10 μg of membranes prepared from CHO-K1 cells stably expressing 5-HT1A receptors were incubated with indicated concentrations of [3H]5-HT in the absence (total binding) or presence of 1000-fold access unlabeled Serotonin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

  • CHO-K1/5-HT1A/Gα15 Stable Cell Line
  • CHO-K1/5-HT1A/Gα15 Stable Cell Line

    Figure 3 10 μg of membranes prepared from CHO-K1 cells stably expressing 5-HT1A receptors were incubated with indicated concentrations of 5-HT in the presence of 2 nM [3H]5-HT. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.


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