Figure 1. Glucagon-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GCGR/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist glucagon. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Glucagon (Mean ± SD, n = 2). The EC50 of Glucagon on this cell was 0.46 µM.
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50 -X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/GCGR/Gα15 Stable Cell Line
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