Catalog Products » Stable Cell Lines » Human Recombinant Gonadotropin-releasing Hormone Receptor Stable Cell Line
CHO-K1/GNRHR/Gα15 Stable Cell Line

Figure 1. Buserelin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GNRHR/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist buserelin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of buserelin (Mean ± SD, n = 2). The EC50 of buserelin on this cell was 20.2 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/GNRHR/Gα15 Stable Cell Line

The gonadotropin-releasing hormone receptor (GNRHR), also known as the luteinizing hormone releasing hormone receptor (LHRHR), is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Upon activation, the LHRHr stimulates tyrosine phosphatase and elicits the release of LH from the pituitary. Evidence exists showing the presence of LHRH and its receptor in extrapituitary tissues as well as a role in progression of some cancers.
M00426
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Description

The gonadotropin-releasing hormone receptor (GNRHR), also known as the luteinizing hormone releasing hormone receptor (LHRHR), is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Upon activation, the LHRHr stimulates tyrosine phosphatase and elicits the release of LH from the pituitary. Evidence exists showing the presence of LHRH and its receptor in extrapituitary tissues as well as a role in progression of some cancers.

Synonyms

GnRH receptor, luteinizing hormone-releasing hormone (LHRH) receptor, LHRHR, LRHR, GRHR, GNRHR1, luliberin receptor, GnRH1, Type I GnRHR, LHRH receptor, GnRH I receptor, GH1, gnRH receptor, gnRH-R, gonadotropin-releasing hormone receptor, growth hormone

Overview
Applications Functional assay for GNRHR receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/GNRHR/Gα15 Stable Cell Line
  • CHO-K1/GNRHR/Gα15 Stable Cell Line

    Figure 1. Buserelin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GNRHR/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist buserelin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of buserelin (Mean ± SD, n = 2). The EC50 of buserelin on this cell was 20.2 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom


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