Catalog Products » Stable Cell Lines » Human Recombinant Formylpeptide Receptor 1 Stable Cell Line
CHO-K1/FPR1/Gα15 Stable Cell Line

Figure 1. FMLP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/FPR1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist FMLP. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of FMLP (Mean ± SD, n = 2). The EC50 of FMLP on this cell was 0.97 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/FPR1/Gα15 Stable Cell Line

The formyl peptide receptors (FPR) are a class of G protein-coupled receptors involved in chemotaxis. FPR1 is a Gi-coupled GPCR. FPR1 is expressed in the vasculature, secretory epithelial cells, neurons and other tissues. FPR is involved in host defense against bacterial infection and in the clearance of damaged cells.
M00429
$8,500.00

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Description

The formyl peptide receptors (FPR) are a class of G protein-coupled receptors involved in chemotaxis. FPR1 is a Gi-coupled GPCR. FPR1 is expressed in the vasculature, secretory epithelial cells, neurons and other tissues. FPR is involved in host defense against bacterial infection and in the clearance of damaged cells.

Synonyms

FPR1, FMLP, NFPR, FMLPR, Formyl peptide, fMLP, FPR, Fpr1_predicted, LOC292409, fMet-Leu-Phe receptor, formyl peptide receptor 1, fMLF-R

Overview
Applications Functional assay for FPR1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/FPR1/Gα15 Stable Cell Line
  • CHO-K1/FPR1/Gα15 Stable Cell Line

    Figure 1. FMLP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/FPR1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist FMLP. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of FMLP (Mean ± SD, n = 2). The EC50 of FMLP on this cell was 0.97 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


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