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CHO-K1/GPR68/Gα15 Stable Cell Line

Synonyms GPR68; MGC111379; OGR1
Description The GPR68 is a proton-sensing receptor involved in pH homeostasis. A study revealed that in osteosarcoma cells and primary human osteoblast precursors which expression of GPR68 exhibit strong PH-dependent inositol phosphate formation.
Species Human
Culture Medium Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418
Freeze Medium 45% culture medium, 45% FBS, 10% DMSO

Storage Liquid nitrogen immediately upon delivery

Functional assay for GPR68 receptor

  • CHO-K1/GPR68/Gα15 Stable Cell Line
  • CHO-K1/GPR68/Gα15 Stable Cell Line

    H+-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GPR68/Gα15. The cells were loaded with Calcium-4 prior to stimulation with a GPR68 receptor agonist, H+. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of H+ (Mean ± SD, n = 2). The EC50 of H+ on GPR68 co-expressing with Gα15 in CHO-K1 cells was pH 6.29 (n=1 test) and pH6.66 (n=2 test).

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Cat. No. M00439
2 vials $8500.00