Figure 1. Orphanin FQ-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with Orphanin FQ. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Orphanin FQ (Mean ± SEM, n = 3). The EC50 of Orphanin FQ on this cell was 236.1 nM.
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/OPRL1/Gα15 Stable Cell Line
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