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CHO-K1/OPRL1/Gα15 Stable Cell Line

Figure 1. Orphanin FQ-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with Orphanin FQ. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Orphanin FQ (Mean ± SEM, n = 3). The EC50 of Orphanin FQ on this cell was 236.1 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/OPRL1/Gα15 Stable Cell Line

Following the cloning of the classical opioid receptors (mu, delta and kappa), the opiate receptor like-1 (ORL1) was identified as a G-protein coupled receptor (GPCR) with 65% structure homology to the other members of the opioid family. OPRL1 is a receptor for the 17 aa neuropeptide nociceptin/orphanin FQ and may be involved in the regulation of numerous brain activities, particularly instinctive and emotional behaviors. Recently, new study results are consistent with the reported high density of ORL1 receptor mRNA in dorsal raphe nucleus and with inhibitory actions of nociceptin in cells expressing ORL1.
M00447
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Description

Following the cloning of the classical opioid receptors (mu, delta and kappa), the opiate receptor like-1 (ORL1) was identified as a G-protein coupled receptor (GPCR) with 65% structure homology to the other members of the opioid family. OPRL1 is a receptor for the 17 aa neuropeptide nociceptin/orphanin FQ and may be involved in the regulation of numerous brain activities, particularly instinctive and emotional behaviors. Recently, new study results are consistent with the reported high density of ORL1 receptor mRNA in dorsal raphe nucleus and with inhibitory actions of nociceptin in cells expressing ORL1.

Synonyms

NOP, LY322, N/OFQ receptor, ORL1, OP4, NOCIR, OOR, KOR-3, LC132 receptor-like, orphanin FQ receptor, kappa3-related opioid receptor, KOR3, LC132, MOR-C, OFQR, Oprl, XOR1, ROR-C, kappa-type 3 opioid receptor, nociceptin receptor, nociceptin receptor ORL1, opiate receptor-like 1, opioid receptor-like, orphanin FQ receptor-a, orphanin FQ receptor-b, orphanin FQ receptor-e, peptide receptor, seven transmembrane G protein-coupled receptor, morc, NOP-r, nociceptin/orphanin FQ receptor

Overview
Applications Functional assay for OPRL1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/OPRL1/Gα15 Stable Cell Line
  • CHO-K1/OPRL1/Gα15 Stable Cell Line

    Figure 1. Orphanin FQ-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with Orphanin FQ. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Orphanin FQ (Mean ± SEM, n = 3). The EC50 of Orphanin FQ on this cell was 236.1 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom


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