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Concentration-dependent stimulation of intracellular calcium mobilization by GLP-1 (7-37) in CHO-K1/GLP1/Gα15 cells
Figure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a GLP1 receptor agonist, GLP-1 (7-37). The intracellular calcium change was measured by FlexStation. The % stimulation was plotted against the log of the cumulative doses (5-fold dilution) of GLP-1 (7-37) (Mean ± SD, n = 2). The EC50 of GLP-1 (7-37) on GLP1 co-expressing with Gα15 in CHO-K1 cells was 295 nM. The S/B of GLP-1 (7-37) on GLP1 co-expressing with Gα15 in CHO-K1 cells was 18.
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