×
Catalog Products » Other Products » Stable Cell Lines » Human Recombinant Glucagon-like Peptide 1 Receptor Stable Cell Line

CHO-K1/GLP1/Gα15 Stable Cell Line


Synonyms Human Recombinant Glucagon-like Peptide 1 Receptor Stable Cell Line
Description GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning.
Species Human
Culture Medium Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B
Freeze Medium 45% culture medium, 45% FBS, 10% DMSO

Storage Liquid nitrogen immediately upon delivery

CHO-K1/GLP1/Gα15 Stable Cell Line
CHO-K1/GLP1/Gα15 Stable Cell Line

Concentration-dependent stimulation of intracellular calcium mobilization by GLP-1 (7-37) in CHO-K1/GLP1/Gα15 cells
Figure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a GLP1 receptor agonist, GLP-1 (7-37). The intracellular calcium change was measured by FlexStation. The % stimulation was plotted against the log of the cumulative doses (5-fold dilution) of GLP-1 (7-37) (Mean ± SD, n = 2). The EC50 of GLP-1 (7-37) on GLP1 co-expressing with Gα15 in CHO-K1 cells was 295 nM. The S/B of GLP-1 (7-37) on GLP1 co-expressing with Gα15 in CHO-K1 cells was 18.


Cat. No.
Product Name
Size
Price
Quantity
RP10769-0.5 Glucagon-Like Peptide (GLP) II, human
0.5 mg
$90.00
RP10770-5 Glucagon (19-29), human
5 mg
$95.00
RP10770-1 Glucagon (19-29), human
1 mg
$24.00
RP10773-0.5 Glucagon-Like Peptide (GLP) I (7-36), amide, human
0.5 mg
$90.00
RP10872-0.5 Exendin (9-39)
0.5 mg
$138.00
RP10874-0.5 Exendin-4
0.5 mg
$147.00
RP12738-0.5 Glucagon-Like Peptide (GLP) I (7-37)
0.5 mg
$119.00
A01826-40 GLP1R Antibody, mAb, Mouse
40 ug
$99.00
 


Notice there is no results found, you can visit "Citations Database" for other results.






For more documents, please visit "Technical Support".
Cat. No. M00451
Size
Price
2 vials $8500.00
 
*
*
*
*