Catalog Products » Stable Cell Lines » Human Recombinant Prostanoid Receptor DP1 Stable Cell Line
CHO-K1/DP1/Gα15 Stable Cell Line

Figure 1. PGD2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/DP1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a DP1 receptor agonist, PGD2. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PGD2 (Mean ± SD, n = 2). The EC50 of PGD2 on this cell was 18.5 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/DP1/Gα15 Stable Cell Line

DP1 is a Gs-coupled GPCR expressed in the colon, eye, platelets, eosinophils, that bind to PGD2 and PGE2. DP1 can inhibit platelet aggregation, and also inhibit leukotriene B4 and superoxide anion (O2-) release from human neutrophils. It can relax smooth muscle and regulate eosinophil apoptosis. DP receptor knockout OVA-challenged mice exhibit reduced Th2 cytokine levels and lymphocyte accumulation in the lung. In addition, they failed to produce asthmatic responses.
M00453
$8,500.00

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Description

DP1 is a Gs-coupled GPCR expressed in the colon, eye, platelets, eosinophils, that bind to PGD2 and PGE2. DP1 can inhibit platelet aggregation, and also inhibit leukotriene B4 and superoxide anion (O2-) release from human neutrophils. It can relax smooth muscle and regulate eosinophil apoptosis. DP receptor knockout OVA-challenged mice exhibit reduced Th2 cytokine levels and lymphocyte accumulation in the lung. In addition, they failed to produce asthmatic responses.

Synonyms

DP1 receptor, prostaglandin D, DP, DP1, PTGDR1, Ptgdr, Ptgdr2, Ptgdrl, PGD receptor-like, PGD2 receptor-like, prostaglandin D receptor, prostaglandin D receptor-like, prostaglandin D2 receptor, prostaglandin D2 receptor-like, prostanoid DP receptor-like

Overview
Applications Functional assay for DP1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/DP1/Gα15 Stable Cell Line
  • CHO-K1/DP1/Gα15 Stable Cell Line

    Figure 1. PGD2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/DP1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a DP1 receptor agonist, PGD2. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PGD2 (Mean ± SD, n = 2). The EC50 of PGD2 on this cell was 18.5 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom


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