Catalog Products » Stable Cell Lines » Human chemokine (C-C motif) receptor 1 (CCR1) Stable Cell Line
CHO-K1/Gα15/CCR1

Figure Dose dependent stimulation of intracellular calcium mobilization upon treatment with its ligand MIP-1α. The CCR1-expressing stable subline was loaded with Calcium-4 prior to being stimulated with a CCR1 receptor agonist, human MIP-1α (GenScript, Cat No: Z03137). The intracellular calcium mobilization was monitored by FLIPR. P1, P5, P15, and P20 represent the cell passage number.

CHO-K1/Gα15/CCR1

C-C chemokine receptor type 1 (CCR1) is a G protein-coupled receptor member of the CC chemokine subfamily of receptors. Following interaction with its specific chemokine ligands, like macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), and monocyte chemoattractant protein 3 (MCP-3), it triggers the onset of a process known as chemotaxis. GenScript’s human CCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay.
M00522
$8,500.00

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Description

C-C chemokine receptor type 1 (CCR1) is a G protein-coupled receptor member of the CC chemokine subfamily of receptors. Following interaction with its specific chemokine ligands, like macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), and monocyte chemoattractant protein 3 (MCP-3), it triggers the onset of a process known as chemotaxis. GenScript’s human CCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay.
Synonyms

CD191; CKR-1; CKR1; CMKBR1; HM145; MIP1aR; SCYAR1

Overview
Applications Functional assay for CCR1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon receiving
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 3 µg/ml puromycin (Cat. #A11138-03, Gibco), 100 µg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/Gα15/CCR1
    • CHO-K1/Gα15/CCR1

    Figure Dose dependent stimulation of intracellular calcium mobilization upon treatment with its ligand MIP-1α. The CCR1-expressing stable subline was loaded with Calcium-4 prior to being stimulated with a CCR1 receptor agonist, human MIP-1α (GenScript, Cat No: Z03137). The intracellular calcium mobilization was monitored by FLIPR. P1, P5, P15, and P20 represent the cell passage number.


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