Catalog Products » Stable Cell Lines » Human chemokine (C-C motif) receptor 5 (CCR5) Stable Cell Line
CHO-K1/Gα15/CCR5

Figure Dose dependent stimulation of intracellular calcium mobilization upon treatment with its ligand MIP-1α. The CCR5-expressing stable subline was loaded with Calcium-4 prior to being stimulated with a CCR5 receptor agonist, human MIP-1α (GenScript, Cat No: Z03137). The intracellular calcium mobilization was monitored by FLIPR. P1, P5, P10, and P20 represent the cell passage number.

CHO-K1/Gα15/CCR5

C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay.
M00523
$8,500.00

Ask us a question
Description

C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay.

Synonyms

CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Overview
Applications Functional assay for CCR5 receptor

Product Introduction
Storage Liquid nitrogen immediately upon receiving
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s) (Cat. #21127, Life Technologies), 10% FBS, 3 µg/ml puromycin (Cat. #A11138-03, Gibco), 100 µg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/Gα15/CCR5
  • CHO-K1/Gα15/CCR5

    Figure Dose dependent stimulation of intracellular calcium mobilization upon treatment with its ligand MIP-1α. The CCR5-expressing stable subline was loaded with Calcium-4 prior to being stimulated with a CCR5 receptor agonist, human MIP-1α (GenScript, Cat No: Z03137). The intracellular calcium mobilization was monitored by FLIPR. P1, P5, P10, and P20 represent the cell passage number.


feedback

Do you like the current new website?

Hate

Dislike

Neutral

Like

Love

*