CHO-K1/PD-1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human programmed cell death 1 (PD-1) on the surface. The surface expression of PD-1 is validated by FACS analysis.

Price	$8500.00
Cat. No.	M00529
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human programmed cell death 1 (PD-1) on the surface. The surface expression of PD-1 is validated by FACS analysis.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

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Figure 1. FACS analysis of cell surface expression of human PD-1 on CHO-K1/PD-1 cells. The CHO-K1/PD-1 cells (blue) and the negative control CHO-K1 cells (red) were probed using APC-conjugated anti-human CD279 (Cat. No. 329908, Biolegend).

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Citations

1. Tanapati Phakham, et al. Highly efficient hybridoma generation and screening strategy for anti-PD-1 monoclonal antibody development. Sci Rep. (2022-10)