Catalog Products » Stable Cell Lines » HEK293/Gα15 Stable Cell Line
Manual
COAs

HEK293/Gα15 Stable Cell Line

HEK293/Gα15 is a HEK293 cell line stably expressing the Gα15 alpha subunit protein which a Gq protein.  It is used as a host cell for transfection expression of Gs and Gi/o -coupled receptors, the constitutively expressed Gα15 protein in the cells allows many transfected receptors which normally stimulate/inhibit the cAMP pathway, to couple to Gq signal transduction and mobilize intracellular calcium.  The cell line carries the puromycin resistance gene and is resistant to puromycin
$8500.00
M00554

Ask us a question
Product Introduction
Product Description Recombinant HEK293 cells stably overexpress human G protein subunit alpha 15 (Gα15) in the cytoplasm. This protein enables G protein - coupled receptor binding activity and acts upstream of or within with a positive effect on calcium - mediated signaling.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Back

Culture Conditions
Culture Medium DMEM, 10% FBS, 0.5 μg/ml Puromycin B (Cat. No. A11138, Life Technologies)
Complete Growth Medium DMEM (Cat. No. 10566, Life Technologies), 10% FBS
Freeze Medium-DATA 90% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)
Back

HEK293/Gα15 Stable Cell Line

Figure 1. Vasoactive intestinal peptide (VIP)-induced stimulation of intracellular calcium mobilization in HEK293/Gα15/VPAC2 cell pool. The cell pool was generated by transient transfection of VPAC2 plasmid on HEK293/Gα15 cells. Then cell pool were loaded with Calcium-4 prior to being stimulated with VAPC2 receptor’s agonist VIP. The intracellular calcium change was measured by FLIPR (Molecular Devices).

»

<
>

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.