Figure 1. AM (1-52)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1 /Gα15/AMY2 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an AMY2 receptor agonist, AM (1-52). The intracellular calcium change was measured by FLIPR. Fluorescence signal were normalized and plotted against the log of the cumulative doses (5-fold dilution) of AM (1-52) (Mean ± SD, n = 4). The EC50 of AM (1-52) on this cells was 34 nM.
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/Gα15/AMY2 Stable Cell Line
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