Catalog Products » Stable Cell Lines » Human Recombinant CD64 Stable Cell Line
CHO-K1/CD64 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of CD64 on CHO-K1/CD64 cells. The CHO-K1/CD64 cells (red) and the negative control CHO-K1 cells (green) were probed using PE-mouse anti-human CD64 (Cat. No. 561926, BD Bioscience).

CHO-K1/CD64 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human high affinity Fc fragment of IgG receptor Ia (FcγRI, CD64) and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of CD64 is validated by FACS analysis. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD64 during biologics research and development.
M00588
$8925.00

Ask us a question
Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human high affinity Fc fragment of IgG receptor Ia (FcγRI, CD64) and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of CD64 is validated by FACS analysis. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD64 during biologics research and development.
Culture Properties Adherent.
Stability Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 300 μg/ml Hygromycin B (Cat. No.10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

Examples
  • CHO-K1/CD64 Stable Cell Line
    • CHO-K1/CD64 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of CD64 on CHO-K1/CD64 cells. The CHO-K1/CD64 cells (red) and the negative control CHO-K1 cells (green) were probed using PE-mouse anti-human CD64 (Cat. No. 561926, BD Bioscience).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.