Catalog Products » Stable Cell Lines » Human Recombinant CD64 Stable Cell Line
CHO-K1/CD64 Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD64 on CHO-K1/CD64 cells.
The CHO-K1/CD64 cells (blue) and the negative control CHO-K1 cells (red) were stained with PE Mouse anti-Human CD64 (Cat. No. 558592, BD Bioscience).

CHO-K1/CD64 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress high affinity human Fc fragment of IgG receptor Ia (FcγRI, CD64) on the cell surface, containing one alpha chain and two gamma chains. The surface expression of CD64 is validated by FACS analysis. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD64 during biologics research and development.
M00588
$8,925.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress high affinity human Fc fragment of IgG receptor Ia (FcγRI, CD64) on the cell surface, containing one alpha chain and two gamma chains. The surface expression of CD64 is validated by FACS analysis. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD64 during biologics research and development.
Applications Cell-based binding assay; use as immunogen for antibody development

Product Introduction
Expressed Gene Codon Optimized from NM_000566.3 (for FCGR1A); Codon Optimized from NM_004106.2 (for FCER1G); No expressed tags.
Host Cell CHO-K1
Quantity 2 vials of frozen cells (>1×106 per vial in 1 ml)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Culture Medium Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 300 µg/ml Hygromycin B (Cat. No.10687010, Invitrogen), 8 μg/ml Puromycin (Cat. No.A11138-03, Gibco)

Background
Gene Synonyms FCRI, CD64A, FCGR1, FCGR1A (for Fc Fragment of IgG receptor Ia); FCER1G, FCRG (for Fc fragment of IgE receptor Ig)
Background CD64 (Cluster of Differentiation 64) is a type of integral membrane glycoprotein, also known as Fc-gamma receptor 1 (FcγRI). It is composed of two subunits- an alpha subunit encoded by FCGR1A (Fc Fragment of IgG Receptor Ia), and a gamma subunit encoded by FCER1G (Fc Fragment of IgE Receptor Ig). It binds monomeric IgG-type antibodies with high affinity. After binding IgG, CD64 interacts with an accessory chain known as the common γ chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellular activation. While CD64 is constitutively found only on macrophages and monocytes, treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. 

Examples
  • CHO-K1/CD64 Stable Cell Line
    • CHO-K1/CD64 Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of CD64 on CHO-K1/CD64 cells.
    The CHO-K1/CD64 cells (blue) and the negative control CHO-K1 cells (red) were stained with PE Mouse anti-Human CD64 (Cat. No. 558592, BD Bioscience).


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