Catalog Products » Stable Cell Lines » Human Recombinant CD32C 13Gln Stable Cell Line
CHO-K1/CD32C 13Gln Stable Cell Line

Figure 1. FACS analysis of cell surface expression of CD32C 13Gln on CHO-K1/CD32 C 13Gln cells. The CHO-K1/CD32 C 13Gln cells (blue) and the negative control CHO-K1 cells (red) were probed using APC-conjugated mouse anti-human CD32 antibody (Cat. No. 559769, R&D Systems).

CHO-K1/CD32C 13Gln Stable Cell Line

Recombinant CHO-K1 cells stably overexpress Homo sapiens cell-type natural killer cells Fc gamma receptor IIc1 (FcγRIIc / CD32C) on the cell surface. The surface expression of FcγRIIc is validated by FACS analysis. This stable cell line is recommended for evaluating the binding affinity between FcγRIIc and Fc region of antibodies, Fc fusion proteins, or antibody-based biologics.
M00601
$8925.00

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Product Introduction
Product Description Recombinant CHO - K1 cells stably overexpress human cell - type natural killer cells Fc gamma receptor IIc1 (FcγRIIc/CD32C) on the surface. The surface expression of CD32C 13Gln is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32C 13Gln. GenScript also offers other CD32 stable cell line for FcγRII polymorphism study.
Culture Properties Adherent.
Stability Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

Examples
  • CHO-K1/CD32C 13Gln Stable Cell Line
    • CHO-K1/CD32C 13Gln Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of CD32C 13Gln on CHO-K1/CD32 C 13Gln cells. The CHO-K1/CD32 C 13Gln cells (blue) and the negative control CHO-K1 cells (red) were probed using APC-conjugated mouse anti-human CD32 antibody (Cat. No. 559769, R&D Systems).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.