Catalog Products » Stable Cell Lines » Human Recombinant CD16B Stable Cell Line
CHO-K1/CD16B NA1 Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD16B NA1 on CHO-K1/CD16B NA1 cells.
The CHO-K1/CD16B NA1 cells (blue) and the negative control CHO-K1 cells (blue) were stained with PE anti-human CD16 antibody (Cat. No. 302008, BioLegend).

CHO-K1/CD16B NA1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb / CD16B NA1). The surface expression of CD16B NA1 is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16B during biologics research and development.
M00602
$8,925.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb / CD16B NA1). The surface expression of CD16B NA1 is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16B during biologics research and development.
Applications Cell-based binding assay; use as immunogen for antibody development

Product Introduction
Expressed Gene Codon optimized from CCDS41433.1; no expressed tags
Target Protein NP_000561.3
Host Cell CHO-K1
Quantity 2 vials of frozen cells (>1×106 per vial in 1 ml) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Culture Medium Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

Background
Gene Synonyms CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
Background CD16 has been identified as FcγRIIIa (CD16A) and FcγRIIIb (CD16B) which are similar to each other. CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells. CD16B exists as a monomeric glycosylphosphatidylinositol (GPI)-anchored form in neutrophils [1]. CD16B (FcγRIIIb) exists in two allelic forms, NA1 and NA2, [2-3]. The polymorphism of CD16B exhibits different binding affinities for IgG1 and IgG3 subclasses [3]. The different allelic versions are also demonstrated to be associated with susceptibility to diseases like idiopathic pulmonary fibrosis [4] and systemic lupus [5].

Examples
  • CHO-K1/CD16B NA1 Stable Cell Line
  • CHO-K1/CD16B NA1 Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of CD16B NA1 on CHO-K1/CD16B NA1 cells.
    The CHO-K1/CD16B NA1 cells (blue) and the negative control CHO-K1 cells (blue) were stained with PE anti-human CD16 antibody (Cat. No. 302008, BioLegend).


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