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CHO-K1/FcRn Stable Cell Line

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Figure 1: FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells.
The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were stained with anti-FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz) and PE conjugated Goat anti-mouse IgG (minimal cross-reactivity) (Cat. No. 405307, BioLegend).

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Overview

Product Description

Recombinant CHO-K1 cells stably overexpress human neonatal Fc receptor (FcRn) and beta-2 microglobulin (B2M). The existence of B2M enables the stable expression of FcRn. The surface expression of FcRn is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, and antibody based biologics that binds with FcRn during biologics research and development.

Applications

Cell-based binding assay; use as immunogen for antibody development

Product Introduction

Expressed Gene	Codon optimized from NM_004107 (for FcRn); codon optimized from NM_012673 (for B2M); no expressed tags
Target Protein	NP_004098.1 (for FcRn); NP_036805.1 (for B2M)
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

Culture Conditions

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No.D2650, Sigma)
Complete Growth Medium	Ham's F-12K (Kaighn's) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	Ham's F-12K (Kaighn's) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin(Cat. No. A11138-03, Gibco), 400 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

Background

Gene Synonyms	FCGRT (for FcRn); beta-2-Microglobulin (for B2M)
Background	This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation.