Figure 1: FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells.
The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were stained with anti-FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz) and PE conjugated Goat anti-mouse IgG (minimal cross-reactivity) (Cat. No. 405307, BioLegend).
Product Description | Recombinant CHO-K1 cells stably overexpress human neonatal Fc receptor (FcRn) and beta-2 microglobulin (B2M). The existence of B2M enables the stable expression of FcRn. The surface expression of FcRn is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, and antibody based biologics that binds with FcRn during biologics research and development. |
Applications |
Cell-based binding assay; use as immunogen for antibody
development |
Expressed Gene |
Codon
optimized from NM_004107 (for
FcRn); codon optimized from NM_012673 (for B2M); no expressed tags |
Target Protein |
NP_004098.1 (for FcRn); NP_036805.1 (for B2M) |
Host Cell |
CHO-K1 |
Quantity |
2
vials of frozen cells (>1×106 per vial in 1 ml) |
Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status |
Negative.
The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza). |
Culture Properties |
Adherent |
Freeze Medium |
95% complete growth medium,
5% DMSO (Cat. No.D2650, Sigma) |
Complete Growth Medium |
Ham's F-12K (Kaighn's) (Cat.
No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
Culture Medium |
Ham's F-12K (Kaighn's) (Cat.
No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml
Puromycin(Cat. No. A11138-03, Gibco), 400 μg/ml Hygromycin B (Cat. No. 10687010,
Invitrogen) |
Gene Synonyms |
FCGRT (for FcRn); beta-2-Microglobulin (for B2M) |
Background |
This
gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G.
The encoded protein transfers immunoglobulin G antibodies from mother to fetus
across the placenta. This protein also binds immunoglobulin G to protect the
antibody from degradation. |
Figure 1: FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells.
The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were stained with anti-FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz) and PE conjugated Goat anti-mouse IgG (minimal cross-reactivity) (Cat. No. 405307, BioLegend).
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