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GenScript's Precast Gels High resolution & Robust Separation

  • Large well volume – Up to 80 µl for diluted samples
  • High resolution – Even, sharp bands, guaranteed lot-lot consistency
  • Long shelf life – Up to 12 months at 2-8℃
  • Cost effective – 30-50% price reduction compared to other major competitors
  • Compatible cassette design – Fits all popular mini-gel tanks

GenScript's Bis-Tris precast gel series are high performance polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. They also run at neutral pH which minimizes protein modification compared to Tris-glycine gels.

GenScript's gel series include-SurePAGE™ Gels which are the premium, higher resolution and reproducibility gels, and ExpressPlus, good quality and very cost-effective gels.

View the detailed comparison between SurePAGE,
ExpressPlus and other major gel types.

 

  • ExpressPlus (Bis-Tris)

  • SurePAGE (Bis-Tris)

  • Competitor B (Tris-Glycine)

  • Homemade (Tris-Glycine)

  • Competitor T (Bis-Tris)

Free samples of SurePAGE™ and ExpressPlus™ (including MOPS buffer powder) available. Contact us at freesample@genscript.com.

SurePAGE™ Bis-Tris Gels (10x8)

SurePAGE™, Bis-Tris gels are a major upgrade from ExpressPlus™ gels with enhanced casting technology that results in better resolution and consistency.

Superior resolution

  • SurePAGE
    (Bis-Tris)

  • Competitor B (Tris-Glycine)

  • Homemade (Tris-Glycine)

  • Competitor T (Bis-Tris)

Figure 2: SurePAGE™ gels offer superior band resolution compared to competitors and the homemade Tris-Glycine gels. Lane 1 and 5: protein marker (MM1397), 5µl. Lane 2,,3,4,6,7,8 and 9: E. coli 10ul cell lysate.

  • Cost effective

    precast gel
  • Easier loading, Larger well volumes

    precast gel

GenScript Precast gel selection guide

Compatible gel tanks

Bio-Rad Mini-PROTEAN® II & 3*

Bio-Rad Mini-PROTEAN®Tetra System*

Invitrogen Novex XCell I, II, & Surelock® (Use with GenScript Gel Tank Adapter Plates)

LONZA PAGEr® Minigel Chamber

Hoefer Mighty Small (SE 260/SE 250)

Hoefer Tall Mighty Small (SE 280)

*please reverse the gasket, see instructions below

Gel series Well No. Recommended Max. loading V./well Well size Recommended amount of protein/well
10x8 Gels 10 60µl 80µl 60µg
12 45µl 60µl 50µg
15 30µl 40µl 40µg

For best result, we recommend using 4XLDS sample buffer (M00676) as the sample loading buffer.

An alternative sample buffer is 5x SDS sample buffer (MB01015). The figure below shows that compared to SDS sample buffer, LDS sample buffer enables better separation of the protein samples.

 
BUY MORE SAVE MORE
Free MOPS running buffer always included! Buy 10 boxes or more and save $10/box!
Buy 5 units (20x5 gels) or more and save $20/unit!
Resources

ExpressPlus™ 10x8 gel ordering list


Cat. No.
Product Name
Size
Price
Quantity
MB01015 5X Sample Buffer
5 ml
$24.00
M00138 Tris-MOPS-SDS Running Buffer Powder
1 Box (5/PK)
$25.00
M00139 Transfer Buffer Powder
1 Box (10/PK)
$58.00
 


* Price valid on GenScript ExpressPlus? PAGE Gel orders for the US and Canada only. Customers ordering from other countries please contact Product@GenScript.com for the latest pricing information in your region.

** GenScript ExpressPlus™ PAGE Gels are formulated using 1x Bis-Tris buffer (pH 6.8). For best results, please use GenScript running buffer (Cat. No. M00138) for gel running, and GenScript transfer buffer (Cat. No. M00139) for protein transfer after running the ExpressPlus™ PAGE gels. Otherwise, please prepare buffers according to our protocol.

Protein Migration Table

The Protein Migration Table below helps you choosing the appropriate gels for protein electrophoresis. The data of this table is built on electrophoresis at 10 volts for 55 min using ExpressPlus™ PAGE gels.

10x8 Gel

precast gel

Cat. No.
Product Name
Size
Price
Quantity
L00671 Tank Adaptor
2 pcs/unit
$12.00
L00674 Cassette Opener
1 kit
$10.00
M00676-10 4X LDS Sample Buffer
10 ml
$10.00
M00676-250 4X LDS Sample Buffer
250 ml
$90.00
M00677 MES SDS Running Buffer Powder
1 5 pcs/box
$25.00
MB01015 5X Sample Buffer
5 ml
$24.00
M00138 Tris-MOPS-SDS Running Buffer Powder
1 Box (5/PK)
$25.00
M00139 Transfer Buffer Powder
1 Box (10/PK)
$58.00
 

*Tank Adaptor(Cat. No. L00671) is for use with Invitrogen Novex XCell I, II, & Surelockreg;

Gel series Document name
SurePAGE™ 10x8 gels Quick Guide
  Manual
  SDS
ExpressPlus™ 10x8 gels Quick Guide
  Manual
  SDS

FAQ

  1. What's the difference between ExpressPlus™ and SurePAGE™ gels?

    SurePAGE™ is a major upgrade from ExpressPlus™ with improved casting technology. SurePAGE™ offers better band resolution and consistency compared to ExpressPlus™ gels.

  2. How long does it take to run a ExpressPlus™ or SurePAGE™ gel?

    It depends on the concentration of the gel. Usually it takes about 50 minutes at 140V. Shorter running times can be achieved if a higher voltage is used, but higher than 180V is not recommended.

  3. What running tanks are ExpressPlus™ and SurePAGE™ gels compatible with?

    Any tank that is designed to run 10cm x 8cm (length x width) precast gels. See product page for the list.

  4. Is this gel stable at room temperature?

    Yes. SurePAGE™ and ExpressPlus™ gels are stable at room temperature for at least three months. However, it is recommended to store gels at 2-8℃ to maintain their highest quality.

  5. Which sample loading buffer should I choose?

    We recommend using 4xLDS sample buffer (M00676) as it gives better sample separation compared to SDS buffer, see product page for detail.

  6. What running buffer should I use to run the gel?

    We recommend MOPS for large and medium proteins, and MES for small proteins. Tris-glycine buffer is NOT compatible with GenScript's precast gels.

  7. What's the buffer I should use to transfer the gel?

    We recommend Tris Bicine transfer buffer (M00139).

  8. Why do some parts of the gel become sticky following protein transfer?

    This is caused by the concentrated polyacrylamide on the top part of the gel. It will help if this part of the gel is cut and removed before transfer.

  9. Will overnight destaining affect the resolution of the band?

    It depends. We suggest using a destaining solution consisting of 15% methanol and 10% acetic acid in DI water for overnight destaining.

Trouble shooting

Problems Probable cause Solution
Distorted protein bands Air bubbles in the sample wells Use a syringe or a pipette to flush the sample wells thoroughly with running buffer before sample loading
Some part of the tracking dye changed to yellow Buffer enters gel because of broken cassette Gel tank is not compatible or cassette was damaged
pH value decreased Prepare new running buffer with deionized water. Check pH
Streaking Insoluble or weakly charged particles (such as carbohydrates) in sample Heat sample in the presence of SDS, centrifuge sample and load the supernatant
Electrophoresis time is too long Seal is not removed from the bottom of the cassette Peel the seal off from the bottom of cassette before loading
Incorrect running conditions Use fixed voltage and automated current, e.g. 140V throughout the electrophoresis
Bands are not well separated Incorrect gel percentage Use the protein migration table to choose the appropriate gel
Sample overloading Reduce sample loading amount, especially when the sample contains many kinds of protein.
Insufficient SDS in loading buffer Increase SDS content in the sample during preparation
Insufficient buffer to keep tank cool Add more buffers to the outer tank until it's at the same level or above the top of the sample wells
Sample spreading across the gel Sample contains too much salt Reduce salt content by dialysis or ultra-filtration
The voltage cannot reach the set value Leaking between the inner and outer tank during run Use compatible gel tank
Excess salt in the sample Reduce salt content by dialysis or ultra-filtration
Lots of air bubbles between the gel and the cassette Running buffer is hot after electrophoresis Add more running buffer to the outer tank
 
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