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GenScript's Bis-Tris precast gel series are high performance polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. They also run at neutral pH which minimizes protein modification compared to Tris-glycine gels.
GenScript's gel series include-SurePAGE™ Gels which are the premium, higher resolution and reproducibility gels, and ExpressPlus, good quality and very cost-effective gels.
View the detailed comparison between SurePAGE,
ExpressPlus and other major gel types.
Free samples of SurePAGE™ (Including MOPS
buffer powder) available. Contact us firstname.lastname@example.org.
SurePAGE™, Bis-Tris gels are a major upgrade from ExpressPlus™ gels with enhanced casting technology that results in better resolution and consistency.
Figure 2: SurePAGE™ gels offer superior band resolution compared to competitors and the homemade Tris-Glycine gels. Lane 1 and 5: protein marker (MM1397), 5µl. Lane 2,,3,4,6,7,8 and 9: E. coli 10ul cell lysate.
*please reverse the gasket, see instructions below
An alternative sample buffer is 5x SDS sample buffer (MB01015). The figure below shows that compared to SDS sample buffer, LDS sample buffer enables better separation of the protein samples.
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*Tank Adaptor(Cat. No. L00671) is for use with Invitrogen Novex XCell I, II, & Surelock®
SurePAGE™ is a major upgrade from ExpressPlus™ with improved casting technology. SurePAGE™ offers better band resolution and consistency compared to ExpressPlus™ gels.
It depends on the concentration of the gel. Usually it takes about 50 minutes at 140V. Shorter running times can be achieved if a higher voltage is used, but higher than 180V is not recommended.
Any tank that is designed to run 10cm x 8cm (length x width) precast gels. See product page for the list.
Yes. SurePAGE™ and ExpressPlus™ gels are stable at room temperature for at least three months. However, it is recommended to store gels at 2-8℃ to maintain their highest quality.
We recommend using 4xLDS sample buffer (M00676) as it gives better sample separation compared to SDS buffer, see product page for detail.
We recommend MOPS for large and medium proteins, and MES for small proteins. Tris-glycine buffer is NOT compatible with GenScript's precast gels.
We recommend Tris Bicine transfer buffer (M00139).
This is caused by the concentrated polyacrylamide on the top part of the gel. It will help if this part of the gel is cut and removed before transfer.
It depends. We suggest using a destaining solution consisting of 15% methanol and 10% acetic acid in DI water for overnight destaining.
Please contact us if you need immediate assistance.
Please contact us (phone: 1-877-436-7274 or email: email@example.com),if you need immediate assistance.