SurePAGE™ and SurePAGE™ Plus Midi Gel Specifications

*Not all concentrations and wells are available online. Please contact your sales representative for details.

*Free samples of SurePAGE™ (Including MOPS buffer powder) available. Contact us [email protected].

Selection Guide

GenScript's high quality mini & midi gels generate the best electrophoresis performance with GenBox Mini Tank and GenBox Midi Tank.

Other compatible gel tanks

• Bio-Rad Mini-PROTEAN^®1 II & 3*
• Bio-Rad Mini-PROTEAN^®1 Tetra System*

*Please reverse the gasket on the inner electrode assembly before use. See manual for detail.

• Thermo SureLock™² Tandem Midi Gel Tank
• Thermo XCell4 SureLock™² Midi-Cell
• Bio-Rad Criterion™¹ Vertical Electrophoresis Cell*
• Bio-Rad Criterion™¹ Dodeca™ Cell*

*A customized adapter is required.

Note:
¹ Registered trademarks of Bio-Rad Laboratories.
² Registered trademarks of Thermo Fisher Scientific.
Disclaimer: These trademarks are used for identification and compatibility purposes only. GenScript products are not affiliated with, sponsored, or endorsed by the aforementioned companies.

Proteins are separated on SurePAGE™ using MOPS or MES running buffer. Tris-glycine buffer is NOT compatible with GenScript's precast gels. Choose the gel that best separates your protein of interest based on the migration chart below.

Gel series	Well No.	Recommended Max. loading V./well	Well size
SurePAGE™	10	60 µl	80 µl
	12	45 µl	60 µl
	15	30 µl	40 µl
SurePAGE™ Plus Midi	20	50 µl	60 µl
	26	30 µl	40 µl
	30	15 µl	30 µl
	12 + 2	90 µl + 30 µl	100 µl + 35 µl

*Protein loading quantity per lane correlates with the specific well volume of the gel. To achieve optimal band resolution, loading quantity should be optimized according to sample type.

For best result, we recommend using 4XLDS sample buffer (M00676) as the sample loading buffer.

An alternative sample buffer is 5x SDS sample buffer (MB01015). The figure below shows that compared to SDS sample buffer, LDS sample buffer enables better separation of the protein samples.

GenScript offers a wide selection of protein molecular weight standards for SDS-PAGE and western blot, including prestained, unstained, Western-viewable, and other standards.

Application Data

• Superior Resolution and Sensitivity

  SurePAGE
  (Bis-Tris)

  

  Competitor B
  (Tris-Glycine)

  

  Competitor T
  (Bis-Tris)

  

  Competitor T
  (Bis-Tris)

  

  Figure 2: SurePAGE™ gels offer superior band resolution and sensitivity.

  Samples:
  • Lane 1, 5: 5μl PAGE-MASTER Protein Standard Plus (GenScript, M001397-500).
  • Lane 2, 3, 4, 6, 7, 8: 10μl E. coli Cell Lysate.

  

  Figure 3: SurePAGE™ Plus Midi gels offer superior band resolution and sensitivity.

  Precast gel: SurePAGE™ Plus Midi, Bis-Tris, 4-12%, 20 wells (GenScript, M01203).

  Samples:

  Lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
  Sample	Protein marker	HEK293 Cell Lysate						Protein marker	Mix 1	Mix 2	Mix 3	Mix 4	Protein marker	E. coli Cell Lysate						Protein marker
  Load Protein (μg)	/	20	10	5	2.5	1.25	0.625	/	see table below				/	20	10	5	2.5	1.25	0.625	/
  Load Volume (μl)	8	20						5	10				8	20						8

  Mix Proteins	Mix 1	Mix 2	Mix 3	Mix 4
  IgG (ng)	2000	1000	500	250
  BSA (ng)	200	100	50	25
  Lysozyme (ng)	800	400	200	100

• High Sample Loading Capacity
  

  Easier loading, Larger well volumes

  

  Figure 4: Unique Well Design of SurePAGE™ Plus Midi gels enable higher sample volume loading.

  Precast gel: SurePAGE™ Plus Midi, Bis-Tris, 4-12%, 12+2 wells (GenScript, M01206).

  Samples:
  • Lane 2, 13: 20μl, Broad Multi Color Pre-Stained Protein Standard (GenScript M00624).
  • Lane 3-12: HEK293 cell lysate loaded sequentially with 60μg/60μL, 50μg/50μL, 40μg/40μL, 30μg/30μL, 20μg/20μL, 10μg/20μL, 5μg/20μL, 2μg/20μL, 1μg/20μL, and 0.5μg/20μL protein.
• Excellent Well-to-well Consistency
  

  Figure 5: 30‑Well SurePAGE™ Plus Midi gels show good band uniformity.

  Precast gel: SurePAGE™ Plus Midi, Bis-Tris, 4-20%, 30 wells (GenScript, M01209).

  Samples:
  • Lane 2, 3, 29: 5μl, Broad Multi Color Pre-Stained Protein Standard (GenScript M00624).
  • Lane 4-28: each well was loaded with 15μL E. coli cell lysate containing 15μg protein.
• Western Blot Performance
  

  Figure 6: The high sensitivity of the SurePAGE™ series precast protein gels, combined with GenScript‘s eBlot™ L2 fast protein transfer system, enables full-range protein transfer.

  Samples: HEK 293 cell lysate with protein amounts of 30μg, 15μg, 7.5μg, 3.75μg, 1.87μg, 0.94μg, 0.47μg, 0.23μg, 0.12μg, 0.06μg.

  Protein transfer: eBlot™ L2 Fast Protein Transfer Device (GenScript, L00981C), standard method.

Customer Testimonials

"Since our throughput is large, SurePAGE Gel with its cost-efficiency and reliable results is more advantageous for us in the long term. SurePAGE gels also have larger well capacity providing excellent convenience for loading more samples." Read More >>

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Li Lab

Stanford University and Arc Institute

"We find the SurePAGE Gels to be the best on the market. The bands are sharper and crisper, while the gels are highly stable, even generating great results after being left at room temperature for several months." Read More >>

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Andrew Lees

Fina BioSolutions LLC

"SurePAGE gel was an instant success for our lab! It showed impressive capability in separating 47kDa and 50kDa proteins with exceptional resolution. Unlike other gels we've tried, the GenScript gel not only achieved excellent separation and resolution but also delivered results of such high quality that could be used in publications." Read More >>

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Dr. Lei Liu

Harvard Medical School

"Our area of interest is in Developmental Biology and we are interested in proteins that are involved in transcriptional regulation, autophagy, mitophagy, apoptosis. GenScript's SurePAGE™ precast gels provides an economical and quality option for our research needs and saves us at least an hour during gel electrophoresis."

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Pragnya Das

Drexel University

Citations

Resources

SurePAGE™ precast gels

High resolution & Robust separation

Free Download

ONE-STOP SOLUTION FOR WESTERN BLOTTING

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FAQ

• 1. What's the difference between ExpressPlus™ and SurePAGE™ gels?

  SurePAGE™ is a major upgrade from ExpressPlus™ with improved casting technology. SurePAGE™ offers better band resolution and consistency, and shorter running time compared to ExpressPlus™ gels.

• 2. How long does it take to run an ExpressPlus™ or SurePAGE™ gel?

  It depends on the gel type and concentration. SurePAGE™ gels can run at 200V for 20 minutes using MES running buffer or 30 minutes using MOPS running buffer. ExpressPlus™ gels run at 140V for 50 minutes.

• 3. What running tanks are ExpressPlus™ and SurePAGE™ gels compatible with?

  GenScript’s gels generate the best performance using GenBox Mini Gel tank and are also compatible with Bio-Rad Mini Gel Tanks (see manual for user instructions). Other gel tanks are not recommended.

• 4. Is this gel stable at room temperature?

  Yes. SurePAGE™ gels are stable at room temperature for at least three months. However, it is recommended to store gels at 2-8℃ to maintain their highest quality.

• 5. Which sample loading buffer should I choose?

  We recommend using 4xLDS sample buffer (Cat. No. M00676) as it gives better sample separation compared to SDS buffer, see product page for detail.

• 6. What running buffer should I use to run SurePAGE™ gels?

  We recommend MOPS for large and medium proteins (greater than 30kDa), and MES for small proteins (smaller than 30kDa). Tris-glycine buffer is NOT compatible with GenScript's precast gels.

• 7. What's the buffer I should use to transfer the gel?

  We recommend Tris Bicine transfer buffer (M00139).

• 8. Will overnight destaining affect the resolution of the band?

  For overnight destaining, we suggest using a weaker destaining solution consisting of 15% methanol and 10% acetic acid in water.

• Trouble shooting

  Problems	Probable cause	Solution
  Distorted protein bands	Air bubbles in the sample wells	Use a syringe or a pipette to flush the sample wells thoroughly with running buffer before sample loading
  Some part of the tracking dye changed to yellow	Buffer enters gel because of broken cassette	Gel tank is not compatible or cassette was damaged
  	Gel wells are not fully submerged with running buffer due to not enough buffer loading or cassette leakage	Add more running buffer in the cathode tank cassette (between the two gels) until it overflows from the gel wells; Press the gel firmly into the cassette to ensure it is fully sealed; Use a functional/not damaged thank cassette
  Streaking	Insoluble or weakly charged particles (such as carbohydrates) exist in the sample	Heat up the sample in the presence of SDS, centrifuge sample and load the supernatant
  Electrophoresis time is too long	Check the specifications of the power supply if it can support multiple gel runs at the same time	Peel the seal off from the bottom of cassette before loading
  	Incorrect running conditions	Use fixed voltage and automated current, e.g. 140V throughout the electrophoresis
  Electrophoresis does not start with error shown on the power supply	Seal is not removed from the bottom of the cassette	Peel the seal off from the bottom of cassette before loading
  Bands are not well separated	Incorrect gel percentage	Use the protein migration table to choose the appropriate gel
  	Sample overloading	Reduce sample loading amount, especially when the sample contains many kinds of protein.
  	Insufficient SDS in loading buffer	Increase SDS content during sample preparation
  	Insufficient buffer to keep tank cool	Add more buffers to the outer tank until it is at the same level or above the top of the sample wells
  Sample spreading across the gel	Sample contains too much salt	Reduce sample salt content by dialysis or ultra-filtration
  The voltage cannot reach the set value	Leaking between the inner and outer tank during run	Use compatible gel tank
  	Run too many gels on the same power supply	Check the specifications of the power supply if it can support multiple gel runs at the same time
  Lots of air bubbles between the gel and the cassette	Overheat might be the cause, especially if running buffer is hot after electrophoresis	Add more running buffer to the outer tank
  Sample do not separate or migrate with a low current	Gel wells are not fully submerged with running buffer due to not enough buffer loading or cassette leakage	Add more running buffer in the cathode tank cassette (between the two gels) until it overflows from the gel wells; press the gel firmly into the cassette to ensure it is fully sealed; use a functional/not damaged tank cassette
  Wide and narrow bands in the gel	Differences in conductivity of the sample might be the cause. If the conductivity of the sample is high (more salt) the bands will become wide and if it is low (less salt) will become narrower.	Adding sample buffer to the blanks and ladder will help to equalize the conductivity and migration.