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Catalog Products » Recombinant Proteins » Cytokines » Tumor Necrosis Factor-alpha (TNF-α), Human

TNF-α, Human

TNF-α, Human

Biological Activity
TNF-alpha, Human (Cat. No. Z01001) stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED50 for this effect is typically 11-50 pg/mL.

TNF-α, Human

2 μg of TNF-α, Human (Cat. No. Z01001) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

TNF-α, Human

GenScript product showed better activity compared to competitor P

TNF-α, Human

GenScript product showed better activity compared to competitor R

Synonyms TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2
Description Tumor Necrosis Factor-alpha (TNF-a) is a homotrimer with a subunit molecular mass of 17.3 kDa. Tumor Necrosis Factor-alpha(TNF-a) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.
Recombinant Human Tumor Necrosis Factor-alpha (TNF-α) produced in E.coli is a single non-glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, rhTNF-α has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Species Human
Source E. coli
Specific Activity ED50 < 30 pg/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 3.3 x 107 units/mg.
Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG
VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI
SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL
GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Measured Molecular Weight 17.3 kDa, observed by reducing SDS-PAGE.
Purity > 98% as analyzed by SDS-PAGE and HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level Less than 0.2 EU/μg determined by LAL test.
Storage Lyophilized recombinant Human Tumor Necrosis Factor-alpha (TNF-α), remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human TNF-α should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Note For research use only

  • TNF-α, Human
  • TNF-α, Human

    Biological Activity
    TNF-alpha, Human (Cat. No. Z01001) stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED50 for this effect is typically 11-50 pg/mL.

  • TNF-α, Human
  • TNF-α, Human

    2 μg of TNF-α, Human (Cat. No. Z01001) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

  • TNF-α, Human
  • TNF-α, Human

    GenScript product showed better activity compared to competitor P

  • TNF-α, Human
  • TNF-α, Human

    GenScript product showed better activity compared to competitor R


Cat. No.
Product Name
Price
Z00404-10
TNF-α (mutant), Human - 10 ug
$85.00
Z00404-50
TNF-α (mutant), Human - 50 ug
$235.00
Z00404-1
TNF-α (mutant), Human - 1 mg
$1400.00
Z02682-50
TNF-α, Human (P. pastoris-expressed) - 50 ug
$95.00
Z02682-10
TNF-α, Human (P. pastoris-expressed) - 10 ug
$49.00
Z02682-1
TNF-α, Human (P. pastoris-expressed) - 1 mg
$625.00
Z02684
TNF-α, His, Mouse - 100 ug
$259.00
Z02685-50
TNF-α, His, Human - 50 ug
$95.00
Z02685-10
TNF-α, His, Human - 10 ug
$49.00
Z02774-5
TNF-α, Mouse - 5 ug
$65.00
Z02774-20
TNF-α, Mouse - 20 ug
$159.00
 
Angelini DJ, et al. TNF{alpha} Increases Tyrosine Phosphorylation of Vascular Endothelial-Cadherin and Opens the Paracellular Pathway Through Fyn Activation in Human Lung Endothelia. Am. J. Physiol. Lung Cell Mol. Physiol. Aug 2006.

Angelone DF, et al. Innate Immunity of the Human Newborn Is Polarized Toward a High Ratio of IL-6/TNF-{alpha} Production In Vitro and In Vivo. Pediatr. Res. Jun 2006.

Frankel SK, et al. TNF-alpha sensitizes normal and fibrotic human lung fibroblasts to Fas-induced apoptosis. Am. J. Respir. Cell Mol. Biol. Mar 2006; 34(3): 293-304.

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Cat. No. Z01001
Size
Price
50 ug $95.00
10 ug $49.00
1 mg $625.00
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