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IL-7, Human

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Product Introduction

Species

Human

Protein Construction

IL-7 (Asp26-His177)
Accession # P13232

Purity

> 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC

Endotoxin Level

< 1 EU/μg of protein by LAL method

Biological Activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine 2E8 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Expression System

E. coli

Theoretical Molecular Weight

17.4 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.

Background

Target Background

Interleukin-7 (IL-7), also known as lymphopoietin 1 and pre-B cell factor, is a hematopoietic growth factor belonging to the IL-7/IL-9 family. It is produced by keratinocytes, dendritic cells, hepatocytes, neurons and epithelial cells. IL-7 binds and signals through IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and common gamma chain receptor. IL-7 plays a role in regulating early B cell and T cell development. It is also important for optimal dendritic cell-T cell interaction.

Synonyms

IL7; IL-7; interleukin 7; Lymphopoietin 1; LP-1; pre-B cell factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.