Catalog Products » Cytokines » IL-1β, Mouse
Manual
Manual
COAs

IL-1β, Mouse

Interleukin-1 Beta (IL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.
$110.00
Z02985-10

Ask us a question
Product Introduction
Species Mouse
Protein Construction

Expressed with an N-terminal Met.

IL-1β (Val118-Ser269)
Accession # P10749
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 10.0 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, corresponding to a specific activity of 1.0 × 108 U/mg..
Expression System E. coli
Apparent Molecular Weight ~17.5 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Back

IL-1β, Mouse

»

IL-1β, Mouse

»

IL-1β, Mouse

Biological Activity
IL-1beta, Mouse (Cat. No. Z02985) stimulates cell proliferation of D10S cells. The ED50 for this effect is less than 2pg/mL. »

<
>

Background
Target Background Interleukin-1 Beta (IL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.
Synonyms IL1B; IL-1; IL1-BETA; IL1F2; interleukin 1 beta; IL1beta
Back

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Jialin Chen 1 2 3 4, et al. Stiffness-dependent dynamic effect of inflammation on keratocyte phenotype and differentiation. Biomed Mater. (2023-04)
Wentao Fan, et al. Estrogen receptor β activation inhibits colitis by promoting NLRP6-mediated autophagy. Cell Rep. (2022-10)