Catalog Products » Cytokines » IFN-γ, Human (CHO-expressed)
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IFN-γ, Human(CHO-expressed)

Human Interferon gamma (hIFN-γ) is amacrophage-activating factor and the lone member of Interferon type II. The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.
$42.00
Z02986-10

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Product Introduction
Species Human
Protein Construction
IFN-γ (Gln24-Gln166)
Accession # P01579
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 2.0 ng/ml, measured in a cytotoxicity assay using HT-29 cells, corresponding to a specific activity of > 5.0 × 105 U/mg.
Expression System CHO
Apparent Molecular Weight 15~25 kDa, on SDS-PAGE under non-reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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IFN-γ, Human(CHO-expressed)

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IFN-γ, Human(CHO-expressed)

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IFN-γ, Human(CHO-expressed)

ED₅₀< 2 ng/ml, measured in a cytotoxicity assay using HT-29 cells, corresponding to a specific activity of > 5×10⁵ units/mg. »

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Background
Target Background Human Interferon gamma (hIFN-γ) is amacrophage-activating factor and the lone member of Interferon type II. The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.
Synonyms Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; IFN-gamma
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Huaguo Huang, et al. Identification of African swine fever virus MGF505-2R as a potent inhibitor of innate immunity in vitro. Virol Sin. (2022-11)