IFN-α 2a, Human

Interferon-Alpha 2a (IFN-Alpha 2a), Human produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2a shares 99.4% , 98.8% aa sequence identity with IFN-alpha 2b and 2c respectively. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.

Price	$42.00
Cat. No.	Z03003-10
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

IFN-α 2a (Cys24-Glu188)
Accession # P01563

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.1 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 1.0 × 10⁷ U/mg.

Expression System

E. coli

Apparent Molecular Weight

~19.2 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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Biological Activity
IFN-alpha 2a, Human (Cat. No. Z03003) induced cytotoxicity of R&D TF-1 cells. The ED₅₀ for this effect is less than 0.1ng/mL. »

2 μg of IFN-α 2a, Human (Cat. No. Z03003) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining. »

Background

Target Background

Synonyms

Leukocyte interferon; B cell interferon; Type I interferon; IFNA2; IFN-a 2a

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Huaguo Huang, et al. Identification of African swine fever virus MGF505-2R as a potent inhibitor of innate immunity in vitro. Virol Sin. (2022-11)
2. Hannah G Abraham, et al. RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers. Front Oncol. (2021-08)
3. Yu-Long Luo, et al. STAT1 participates in the induction of substance P expression in airway epithelial cells by respiratory syncytial virus. Exp Lung Res. (2020-11)
4. Qiang Z,, Zhou ZY,, Peng T,, Jiang PZ, Shi N,, Njoya EM,, Azimova B, Liu WL, Chen WH,, Zhang GL, Wang F., et al. Inhibition of TPL2 by interferon-α suppresses bladder cancer through activation of PDE4D. J Exp Clin Cancer Res. (2018)
5. ChenShao-Ru, et al. In Vitro Study on Anti-Hepatitis C Virus Activity of Spatholobus suberectus Dunn. Molecules. (2016-11)