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Catalog Products » Recombinant Proteins » Growth Factors » Epidermal Growth Factor (EGF), Rat

EGF, Rat

Synonyms Epidermal Growth Factor, Urogastrone, URG
Description Epidermal Growth Factor (EGF) is a cytokine with 53 amino acids, originally found in mouse submaxillary gland. EGF binds to EGF receptors, ErbB1 and B4, and causes them to be dimerized and phosphorylated. The dimerized and phosphorylated EGFR can bind to several intracellular targets, such as phospholipase Cγ and Ras-GTPase-acting protein, and achieve a series of cascade reactions. EGF is involved in the regulation of cell proliferation and differentiation, and is up-regulated during wound healing, accelerating reepitheliazation and increasing tensile strength. It also stimulates neurite outgrowth and increases the uptake of dopamine in the central nervous system. On the other hand, EGF is up-regulated in the glioma cancer, and related to the length of survivals of the patients.
Recombinant rat Epidermal Growth Factor (rrEGF) produced in E. coli is a single non-glycosylated polypeptide chain containing 54 amino acids. A fully biologically active molecule, rrEGF has a molecular mass of 6.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P07522
Species Rat
Source E. coli
Biological Activity ED50 < 0.08 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1.25× 107 units/mg.
Sequence
MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG
ERCQHRDLRW WKLR

Measured Molecular Weight 6.3 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant rat Epidermal Growth Factor (rrEGF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrEGF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




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Cat. No. Z03066
Size
Price
20 ug $25.00
100 ug $75.00
1 mg $400.00