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LIF, Mouse

Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.
$100.00
Z03077-10

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Product Introduction
Species Mouse
Protein Construction
LIF (Ser24-Phe203)
Accession # P09056
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.01 ng/ml, measured by a cell differentiation assay using M1 cells, corresponding to a specific activity of > 1.0 × 108 U/mg.
Expression System E. coli
Apparent Molecular Weight ~19.9 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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LIF, Mouse

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LIF, Mouse

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LIF, Mouse

ED50 < 0.01 ng/ml, measured by a cell differentiation assay using M1 cells, corresponding to a specific activity of > 1.0 × 108 units/mg. »

LIF, Mouse

Lane 1: 2 μg of LIF, Mouse, reducing (R)
Lane 2: 2 μg of LIF, Mouse, non-reducing (NR)
> 95% as analyzed by SDS-PAGE »

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Background
Target Background Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.
Synonyms D factor; CDF; HILDA; D-FACTOR; Differentiation- stimulating factor; Melanoma-derived LPL inhibitor; MLPLI; Emfilermin; Leukemia inhibitory factor; DIA
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Fuquan Chen , et al. Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency. Stem Cell International. (2021-02)
Tingting Gao, et al. Nsd2 Represses Endogenous Retrovirus MERVL in Embryonic Stem Cells. Stem Cells Int. (2021-01)
Fuquan Chen, et al. Histone chaperone FACT represses retrotransposon MERVL and MERVL-derived cryptic promoters. Nucleic Acids Res. (2020-09)
Fu E, et al. Histone demethylase Kdm2a regulates germ cell genes and endogenous retroviruses in embryonic stem cells. Epigenomics. (2019-05)