|Synonyms||CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA|
|Description||Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.
Recombinant mouse Leukemia Inhibitory Factor (rmLIF) produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rmLIF has a molecular mass of 19.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Specific Activity||ED50 < 0.01 ng/mL, measured by a cell differentiation assay using M1 cells, corresponding to a specific activity of > 1.0× 108 units/mg.|
SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL
FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL
VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID
VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK
LGCQLLGTYK QVISVVVQAF SPLPITPVNA TCAIRHPCHG
NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC
APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD
QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG
HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
|Measured Molecular Weight||19.9 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH8.0.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse Leukemia Inhibitory Factor (rmLIF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmLIF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
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