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IL-10, Human(CHO-expressed)

Interleukin-10 (IL-10), initially known as Cytokine Synthesis Inhibitory Factor (CSIF), belongs to the IL-10 family and shares more than 80% sequence homology with Epstein-Barr Virus protein BCRFI. It is produced by many immune cells, such as T-cells, macrophages, mast cells, and dendritic cells. It is usually secreted as a homodimer and, upon binding to its receptor, inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and Th2 cells. It also displays ability to suppress Antigen-Presenting Cell (APC) function. The net effect of Interleukin-10 appears to be inhibitory; however, stimulatory effects, such as stimulation of B cell maturation and antibody production, are also reported.
$100.00
Z03121-10

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Product Introduction
Species Human
Protein Construction
IL-10 (Ser19-Asn178)
Accession # P22301
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 2.0 ng/ml, measured in a cell proliferation assay using MC/9 cells.
Expression System CHO
Apparent Molecular Weight 18~19 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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IL-10, Human(CHO-expressed)

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IL-10, Human(CHO-expressed)

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IL-10, Human(CHO-expressed)

ED50 < 2.0 ng/ml, measured in a cell proliferation assay using MC/9 cells. »

IL-10, Human(CHO-expressed)

Lane 1: 2 μg of IL-10, Human(CHO-expressed), non-reducing (NR)
Lane 2: 2 μg of IL-10, Human(CHO-expressed), reducing (R)
> 95% as analyzed by SDS-PAGE »

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Background
Target Background Interleukin-10 (IL-10), initially known as Cytokine Synthesis Inhibitory Factor (CSIF), belongs to the IL-10 family and shares more than 80% sequence homology with Epstein-Barr Virus protein BCRFI. It is produced by many immune cells, such as T-cells, macrophages, mast cells, and dendritic cells. It is usually secreted as a homodimer and, upon binding to its receptor, inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and Th2 cells. It also displays ability to suppress Antigen-Presenting Cell (APC) function. The net effect of Interleukin-10 appears to be inhibitory; however, stimulatory effects, such as stimulation of B cell maturation and antibody production, are also reported.
Synonyms B-TCGF; CSIF; TGIF
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.