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Catalog Products » Recombinant Proteins » Cytokines » Oncostatin M (OSM), Rat

OSM, Rat

Synonyms OSM
Description Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, which also includes IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and shares a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells.
Recombinant Rat Oncostatin M (rrOSM) produced in E. coli is a single non-glycosylated polypeptide chain containing 215 amino acids. A fully biologically active molecule, rrOSM has a molecular mass of 24.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No Q65Z15
Species Rat
Source E. coli
Biological Activity ED50 < 10 ng/mL, measured by a cell proliferation assay using Rat Embryo Brain cells, corresponding to a specific activity of > 1× 105 units/mg.
Sequence
MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN
TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV
WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL
LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG
YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP
SRSSQSAMLR SLVPR

Measured Molecular Weight 24.5 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE and HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Rat Oncostatin M (rrOSM) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrOSM remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




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Cat. No. Z03144
Size
Price
50 ug $275.00
10 ug $85.00
1 mg $2100.00
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