|Description||Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, which also includes IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and shares a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells.
Recombinant Rat Oncostatin M (rrOSM) produced in E. coli is a single non-glycosylated polypeptide chain containing 215 amino acids. A fully biologically active molecule, rrOSM has a molecular mass of 24.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Specific Activity||ED50 < 10 ng/mL, measured by a cell proliferation assay using Rat Embryo Brain cells, corresponding to a specific activity of > 1× 105 units/mg.|
MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN
TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV
WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL
LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG
YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP
|Measured Molecular Weight||24.5 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE and HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Rat Oncostatin M (rrOSM) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrOSM remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
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