|Synonyms||Human LR3 Insulin-like Growth Factor-I (LR3-IGF-I) (Receptor Grade)|
|Description||IGF-1 is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-1 is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-2, which is also a major fetal growth factor.
Human Long R3 Insulin-like Growth Factor-1 (rhLR3IGF-1) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3)and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs. RhLR3IGF-1 is properly folded under oxidizing conditions and then purified by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 ＜10 ng/ml, measured by the dose-dependantproliferation of CHO cells, corresponding to a specific activity of >1.0× 105 units/mg.|
MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK
PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA
|Measured Molecular Weight||9.1±0.9 KDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE.|
|Formulation||Lyophilized after dissolved in 48%acetonitrile and 0.1% TFA|
|Reconstitution||Reconstituted in 10mM HCl at 1mg/ml. Use a 0.22 μm membrane for filter Sterilization.|
|Endotoxin Level||＜1 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Long R³ Insulin-like Growth Factor-1 (rhLR³ IGF-1) remains stable up to 6 months at lower than -70°C from date of receipt. Stock solution of peptide can be stored at least 3 months at -20°C. Avoid repeated freeze-thaw cycles.|
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