|Description||Glia Maturation Factor beta (GMF-beta) is a 17 kDa brain specific protein that belongs to the ADF/cofilin superfamily. It is a neurotrophin that induces maturation of neurons and glial cells. Unlike other neurotrophins, GMF-β lacks a leader sequence and can be phosphorylated by protein kinase A and protein kinase C suggesting its role in signal transduction. GMF-β is a prominent mediator of inflammation in the central nervous system and can activate several inflammation-related genes such as tumor necrosis factor-α and interleukin-1β. Researchers have shown there are significantly higher levels of GMF-β protein in all the effected regions of Alzheimer’s disease (AD) brains suggesting an important role of GMF-β in AD pathogenesis
Recombinant human Glia Maturation Factor beta (rhGMF-beta) produced in E. coli is a single non-glycosylated polypeptide chain containing 142 amino acids. rhGMF-beta has a molecular mass of 16.7kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||Bioassay data are not available.|
MSESLVVCDV AEDLVEKLRK FRFRKETNNA AIIMKIDKDK
RLVVLDEELE GISPDELKDE LPERQPRFIV YSYKYQHDDG
RVSYPLCFIF SSPVGCKPEQ QMMYAGSKNK LVQTAELTKV
FEIRNTEDLT EEWLREKLGF FH
|Measured Molecular Weight||16.7 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Glia Maturation Factor beta (rhGMF-beta) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhGMF-beta remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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