|Description||Interleukin-6 Receptor (IL-6R) is a single trans-membrane protein that is the receptor for Interleukin-6 (IL-6). IL-6R forms a hexameric complex upon binding 2 molecules of IL-6 and two molecules of glycoprotein 130 (gp130) which activates intracellular JAK/STAT pathways. Although the normal form of IL-6R is the membrane-bound 80 kDa subunit, a soluble form of IL-6R (sIL-6R) can be generated physiologically by limited proteolysis or alternative splicing. sIL-6R binds to both IL-6 and gp130 generating intracellular signaling. In the immune system, sIL-6R is produced by both naïve and memory CD4 T-cells and strongly augments IL-6 ligand’s induction of Th-17 cells.
Recombinant human Soluble IL-6 Receptor Alpha (rhsIL-6R alpha) with an N-terminal His tag produced in Sf9 insect cells is a single glycosylated polypeptide chain containing 350 amino acids. A fully biologically active molecule, rhsIL-6R alpha has a molecular mass of 50kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Source||Sf9 insect cells|
|Biological Activity||ED50 < 50ng/mL, measured by the cytotoxicity assay using M1 cells in presence of 10 ng/mL of human IL-6, corresponding to a specific activity of > 2×104 units/mg.|
HHHHHHDDDD KLAPRRCPAQ EVARGVLTSL PGDSVTLTCP
GVEPEDNATV HWVLRKPAAG SHPSRWAGMG RRLLLRSVQL
HDSGNYSCYR AGRPAGTVHL LVDVPPEEPQ LSCFRKSPLS
NVVCEWGPRS TPSLTTKAVL LVRKFQNSPA EDFQEPCQYS
QESQKFSCQL AVPEGDSSFY IVSMCVASSV GSKFSKTQTF
QGCGILQPDP PANITVTAVA RNPRWLSVTW QDPHSWNSSF
YRLRFELRYR AERSKTFTTW MVKDLQHHCV IHDAWSGLRH
VVQLRAQEEF GQGEWSEWSP EAMGTPWTES RSPPAENEVS
TPMQALTTNK DDDNILFRDS ANATSLPVQD
|Measured Molecular Weight||50 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Soluble IL-6 Receptor Alpha (rhsIL-6R alpha) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhsIL-6R alpha remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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