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Catalog Products » Recombinant Proteins » Cytokines » Melanoma Inhibitory Activity-2 (MIA-2), Human

MIA-2, Human

Synonyms Human Melanoma Inhibitory Activity-2; Human MIA-2
Description Melanoma inhibitory activity protein 2(MIA-2) is a secreted cytokine that is highly expressed in the liver and weakly in the testis. It is a member of the MIA/OTOR family. Members of this family such as MIA, OTOR, and TANGO share a Src homology-3 (SH3)-like domain. Melanoma inhibitory activity 2 is mapped to the gene locus of human chromosome 14q13. MIA and OTOR are exclusively expressed in the cartilage and cochlea, respectively, whereas MIA-2 is expressed exclusively in the liver. MIA-2 expression is transcriptionally regulated by the hepatocyte nuclear factor (HNF)-1-binding site). It is expressed in hepatocellular carcinoma (HCC) but not in bladder, breast, or prostate cancer. MIA-2 inhibits HCC growth and invasion, and consequently acts as a tumor suppressor.
Recombinant Human MIA-2 produced in E. coli cells is a single non-glycosylated polypeptide chain containing 100 amino acids. A fully biologically active molecule, rhMIA-2 has a molecular mass of 11.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No Q96PC5
Source E. coli
Biological Activity Not Available.
Sequence
LESTKLLADL KKCGDLECEA LINRVSAMRD YRGPDCRYLN
FTKGEEISVY VKLAGEREDL WAGSKGKEFG YFPRDAVQIE
EVFISEEIQM STKESDFLCL

Measured Molecular Weight 11.6 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Human MIA-2 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, HumanMIA-2 should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Note For research use only




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Cat. No. Z03337
Size
Price
10 ug $59.00
50 ug $159.00