|Description||Interleukin-22 (IL-22) is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequence, but differ in their biological function. IL-22 is produced by T lymphocytes, and can inhibit IL-4 production by Th2 cells and induce acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-ß/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family. By binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains, IL-22 specifically targets cells of epithelial origin where it induces pro-inflammatory activities.
Recombinant mouse Interleukin-22 (rmIL-22) produced in CHO cells, is a polypeptide chain containing 146 amino acids. It has a molecular mass of 20~30 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Specific Activity||ED50 < 0.5ng/ml, measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells).|
LPVNTRCKLE VSNFQQPYIV NRTFMLAKEA SLADNNTDVR
LIGEKLFRGV SAKDQCYLMK QVLNFTLEDV LLPQSDRFQP
YMQEVVPFLT KLSNQLSSCH ISGDDQNIQK NVRRLKETVK
KLGESGEIKA IGELDLLFMS LRNACV
|Measured Molecular Weight||20~30 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE&HPLC.|
|Formulation||Lyophilized from a 0.2 μm solution in PBS with no additive.|
|Reconstitution||Reconstituted in sterile ddH2O or PBS at 100μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Mouse Interleukin-22 (rmIL-22) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-22 should be stable up to 1 week at 4°C or up to 3 months at -20°C under sterile conditions. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
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