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SARS-CoV-2 Spike protein (ECD, His & Flag Tag)

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. ECD is the extra cellular domain of spike protein.
$380.00
Z03481-100

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Product Introduction
Species SARS-CoV-2
Protein Construction
Spike ECD (Val16-Pro1213)
Accession # P0DTC2		 Poly-His Flag
N-term C-term
Purity > 90% as analyzed by SDS-PAGE
Biological Activity SARS-CoV-2 Spike protein (ECD) can bind with Human ACE2 in functional ELISA assay.
Expression System Sf9 insect cells
Theoretical Molecular Weight 135 kDa
Formulation Supplied as a solution in PBS pH 7.2 containing 10% glycerol.
Concentration Please refer to the COA for the specific lot.
Storage & Stability Upon receiving, this product remains stable for 6 months at -20°C or below. Avoid repeated freeze-thaw cycles.
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SARS-CoV-2 Spike Protein (ECD, His & Flag Tag)

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SARS-CoV-2 Spike Protein (ECD, His & Flag Tag)

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SARS-CoV-2 Spike Protein (ECD, His & Flag Tag)

Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (ECD, His & Flag Tag) (Cat. No. Z03481) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse(Cat.No.A00612) is used as a secondary antibody (0.1 μg/mL). »

SARS-CoV-2 Spike Protein (ECD, His & Flag Tag)

Lane 1: 1μg of SARS-CoV-2 Spike protein (ECD, His & Flag Tag), reducing(R)
> 95% as analyzed by SDS-PAGE »

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Background
Target Background SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. ECD is the extra cellular domain of spike protein.
Synonyms S protein; S-ECD; ECD protein; ECD; 2019-nCoV ECD
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Livija Tu ar; Jure Loboda; Francis Impens; Piotr Sosnowski; Emmy Van Quickelberghe; Robert Vidmar; Hans Demol; Koen Sedeyn; Xavier Saelens; Matej Vizovi ek; Marko Miheli ; Marko Fonovi ; Jaka Horvat; Gregor Kosec; Boris Turk; Kris Gevaert; Du an Turk, et al. Proteomic data and structure analysis combined reveal interplay of structural rigidity and flexibility on selectivity of cysteine cathepsins. Communications Biology. (2023-04)
Yuezhou Chen, et al. Immune recall improves antibody durability and breadth to SARS-CoV-2 variants. Sci Immunol. (2022-05)
Xiangchuan He, et al. A human cell-based SARS-CoV-2 vaccine elicits potent neutralizing antibody responses and protects mice from SARS-CoV-2 challenge. Emerg Microbes Infect. (2021-12)
Ahmad Yanis, et al. Humoral and cellular immune responses to the SARS-CoV-2 BNT162b2 vaccine among a cohort of solid organ transplant recipients and healthy controls. Transpl Infect Dis. (2021-12)
Hsiang-Chi Huang, et al. Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro. EBioMedicine. (2021-11)
Sisi Kang, et al. A SARS-CoV-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation. Nat Commun. (2021-05)
Tingxuan Gu, et al. Cytokine Signature Induced by SARS-CoV-2 Spike Protein in a Mouse Model. Front Immunol. (2021-01)
Chen Y, et al. Quick COVID-19 Healers Sustain Anti-SARS-CoV-2 Antibody Production. Cell. (2020-12)
Thomas Mandel Clausen, et al. SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2. biorxiv. (2020-07-01)