Organism: Human Mouse Rat Other

Pick Primer/Probe Crossing Exon Junction

When Pick Primer/Probe Crossing Exon Junction is selected, the exon regions must be defined. If only raw sequence is provided, the sequence will be mapped on the genome (human, mouse or rat at present) sequences to locate the exon boundaries. Besides, there are other two ways to specify exon regions:

• The best way is to fill in GenBank Accession with the accession number (e.g. NM_145027 , please note the accession number is case-sensitive and should not contain version suffix like '.1' or '.2'). The sequence will be fetched from NCBI data center, and the exon regions are already defined in sequence annotations.
• Manually specify the exon junctions with ':' in the input sequence (e.g. 'ACGCGCG:CGTACG')

Target Nucleotide Sequences:

GenBank Accession:

or Paste in the DNA Sequence in raw format:

Number of Primer/Probe Sets to Return (Limit 20 Sets):

PCR Amplicon Size Range:

Primer Tm: Minimum °C

Optimum

Maximum °C

Probe Tm: Minimum °C

Optimum

Maximum °C