Organism: Human Mouse Rat Other

Pick Primer/Probe Crossing Exon Junction

When Pick Primer/Probe Crossing Exon Junction is selected, the exon regions must be defined. If only raw sequence is provided, the sequence will be mapped on the genome (human, mouse or rat at present) sequences to locate the exon boundaries.

• Manually specify the exon junctions with ':' in the input sequence (e.g. 'ACGCGCG:CGTACG')

Paste in the DNA Sequence in raw format:

Number of Primer/Probe Sets to Return (Limit 20 Sets):

PCR Amplicon Size Range:

Primer Tm: Minimum °C

Optimum

Maximum °C

Probe Tm: Minimum °C

Optimum

Maximum °C