Escherichia coli (E. Coli) is a preferred system for recombinant protein expression because it generally provides the most cost effective solution. However, there are several limitations when using E. Coli for protein expression, include difficultly in production for proteins with strict endotoxin condition requirements and poor secretion for secretory proteins in certain experiments. In order to combat these limitations, GenScript has established a Bacillus subtilis expression platform to produce recombinant proteins with extremely low endotoxin requirement. In this webinar, we will introduce the process of establishment of the expression system, demonstrate its advantages over the traditional E.coli system, and provide some case studies to illustrate the research applications using the B. subtilis expression system.
In this webinar, we will cover:
- How the B. subtilis expression platform was established and how is it used to produce recombinant proteins with extremely low endotoxin requirement.
- We will introduce some case studies to demonstrate the applications that can utilize this B.subtilis system.
Learn more about the customized bacterial protein expression services using B. Subtilis offered by protein expression Experts from GenScript.
Speaker: Dr. Bo Wu, Director, GenScript USA Inc.
Date: Nov. 8th, 2018
Time: 9:00-10:00 AM EST
Dr. Bo Wu is currently the Director of Protein Department at GenScript, USA Inc. In 2007, he graduated, with Honors, from Peking University and received his Bachelor of Science degree in Biological Sciences. He received his Ph.D. degree in Chemistry in 2013, from Ohio State University, where he studied protein and amyloid structure and dynamics using analytical methods in magic angle spinning solid state NMR. During his academic career, Dr. Wu has contributed to 5 research publications as well as presented his research in several academic conferences. In 2013, Dr. Wu joined GenScript USA Inc. as a senior scientist in the Protein Department. Since then, he has been working on product development, project management, as well as R&D methods optimizing mammalian and microbial platforms for recombinant protein and antibody productions.
4 Essential Strategies for Boosting Transient Protein Expression is a free educational and technical resource for scientists in all biomedical disciplines who use proteins or antibodies in their research.
This new application note covers the following 4 "How-to" Strategies:
- Designing an expression vector
- Increasing protein stability
- Utilizing a fusion partner
- Improving purification