Recombinant protein production is not always straightforward. As many factors can impact protein expression and purification, the optimization process is usually labor-intensive and time-consuming. To address some of the uncertainties and challenges, GenScript’s protein expression experts offer custom bacterial expression services with cutting-edge solutions. Now customers can flexibly choose an option that fits their requirements, from codon optimization to gene synthesis to small-scale testing and scale-up, together with protein purification and characterization. In addition, we have developed a specialized protein refolding technology for recovering biologically functional proteins from inclusion bodies, which is available for large-scale manufacturing. High throughput E.coli expression platform offers a cost-effective option for small-scale expression to help expedite your target screening projects.
E.coli is a well-known expression system, easily genetically manipulated, that supports low-cost and high-yield production of a broad range of recombinant proteins, such as cytokines, enzymes, immunogens, and antibody fragments. Our customized workflow can meet your downstream applications. GenScript has successfully delivered more than 50,000 batches of recombinant proteins through E.coli expression system.
| Milestones | Specifications | Deliverables | |
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| 1 | Gene synthesis, Cloning, Plasmid Prep |
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| 2 | Protein Expression Evaluation |
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| 3 | Protein Purification Tag Removal (Optional) Refolding (Optional) |
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| 4 | QC & Delivery |
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| Turnaround time* |
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Note:
*Turnaround times are approximate. In most cases, we deliver within 2-3 weeks. However, optional steps might prolong the overall timeline slightly.
For any large scale protein production requirements, we offer fully customizable E. coli fermentation services. Contact our technical account managers for more details.
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Background
Key Challenge
Optimization
Result
Background
Key Challenge
Optimization
Result
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