Overview

GenScript's BacuVance baculovirus / Insect cell expression system was developed by our in-house team of scientists to enable virus production and expression of recombinant proteins from baculovirus-infected insect cells. This expression system supports improved protein solubility and incorporation of post-translational modifications. It can simultaneously express multiple genes to form heterodimers or polymers (i.e., consisting mostly of 5 subunits) and produce intracellular and secreted proteins at higher yields. Our high throughput insect expression & purification platform is also available for small-scale production to fit your target screening projects.

GenScript can leverage the baculovirus expression system to support the production of difficult-to-express proteins, such as kinases, viral targets, cell surface receptors, transporters, various enzymes, and membrane proteins. As the function of membrane proteins is frequently implicated in disease states, these proteins currently account for over 60% of all drug targets. Despite their biological relevance, membrane protein research is often limited by the challenges of expressing and purifying these proteins. Backed by a team of experts in membrane protein production, GenScript can express and purify membrane proteins while preserving their structural and functional integrity. The BacuVance baculovirus / Insect cell expression system would be highly recommended for protein crystallization and structure determination applications to extend your budget and save time.

Highlights

Outstanding Capability for Complex Protein
Outstanding Capability for Complex Protein
Advanced Expression Optimization Technology
Advanced Expression Optimization Technology
Capacity for large inserts > 400kDa
Capacity for Large Inserts > 400kDa

General Workflow

Protein Insect

Service Details

For customers who wish to establish the best possible protocol for their project and then scale up, GenScript recommends using the sophisticated platform to explore different conditions for the expression of target proteins.

Milestones Specifications Deliverables Timeline1
1 Gene synthesis and subcloning
  • Subcloning into one or more expression vectors
Construct(s) report 2 weeks
2 Virus generation
  • Generation of recombinant Bacmid DNA
  • Transfection of insect cell with recombinant Bacmid DNA
  • Generation of P1 stock (Low titer), P2 stock (High titer), and determination of virus titer by Quantitative-PCR
  • Verify the protein expression for P1 and P2 generation by western blot with anti-His/GST or other antibody
5 ml, >107 pfu/ml virus stock 2–3 weeks
3 Pilot expression evaluation
  • Infection of insect cells with P2 stock
  • One step affinity purification
    SDS-PAGE gel and Western blot
Expression data 1.5 weeks
4 1 L expression and purification
  • 1 L insect cell expression and purification
  • One-step purification
  • QC by SDS-PAGE, Western blot2, and LC-MS/MS coverage2
Purified proteins
QC data
1–2 weeks

Note:
1. Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.
2. Other QC data, such as LC-MS/MS coverage, N-terminal sequencing and LC-MASS are available with additional charge.

Case Studies

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