BacuVance Baculovirus / Insect Cell Expression System

Service Details

For customers who wish to establish the best possible protocol for their project and then scale up, GenScript recommends using the sophisticated platform to explore different conditions for the expression of target proteins.

Note:
1. Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.
2. Other QC tests, such as LC-MS/MS coverage, N-terminal sequencing and LC-MS molecular mass analysis are available with additional charge.

Case Studies

• Case 1: Transmembrane Proteins

  CLDN18.2 with 4 transmembrane domains

  Background

  • Production of CLDN18.2 which is a important drug target for the treatment of many tumors

  Key Challenge

  • Low expression
  • Difficult to purify

  Optimization

  • GOI codon optimization
  • Expression optimization
  • Detergent screening and purification optimization
  • Screening package with 6 detergent
  • In total 11 detergents are available

  Result

  • >2 mg/L yield after optimization
  • Purity >85%
  • EC50 < 0.03 μg/ml

  

  • Since 2020, more than 200 proteins with different transmembrane times have been purified and expressed, with a success rate of 85%.
  • Experienced with membrane proteins with 1, 6 and 7 transmembrane domains

  Hemagglutinin
  (1 transmembrane domain)

  

  LPAR1
  (6 transmembrane domains)

  

  PAQR2
  (7 transmembrane domains)

  
• Case 2: High Molecular Weight Proteins
  • Some high MW proteins, which are insoluble and usually expressed at low levels.
  • A classical insect-baculovirus expression system that can accommodate the insertion of macromolecular fragments is generally used.
  • Hi5 cells successfully expressed high MW protein (100kDa) with a purity of 95% and an expression level of 10mg/L.

  
• Case 3: Kinase
  • Sf9 cell line is a traditional cell line used for baculovirus expression. It has many advantages in protein expression and purification, such as the expressed recombinant protein can be folded correctly and form disulfide bonds as well as accommodate large fragments of foreign genes.
  • Sf9 cells successfully expressed Kinase with a purity of 95% and an expression level of 10mg/L

  
• Case 4: Other Proteins
  • GenScript can provide you with free, advanced codon optimization specific to Sf9, Sf21, Hi-5, and S2 insect cell lines, to further enhance the expression of your protein.

  Virus protein

  

  Multi-subunit complex proteins

  

  Secretory protein