GenScript proudly presents a versatile collection of AAV applied vectors, meticulously designed to streamline your AAV research. These vectors are engineered to deliver superior ITR stability with significantly enhance plasmid production yield. Offering a variety of configurations, they include single-stranded (ssAAV) and self-complementary (scAAV) types, with ITR formats available as either wild-type AAV2 or truncated versions (141 bp and 128 bp) for improved stability and efficient plasmid propagation. Additionally, researchers can choose between two antibiotic resistance options—ampicillin and kanamycin—for greater flexibility.
These vectors are available for bundling with our gene synthesis and cloning service. Order now through our GenSmart 2.0 online ordering system and experience the efficiency and reliability that GenScript delivers for your AAV research needs.
AAV Type | ssAAV, scAAV |
---|---|
ITR Sequence | ITR145, ITR141, ITR128 |
Resistance | Kanamycin, Ampicillin |
Various plasmids containing wild-type AAV2 ITRs were constructed using NEB stable cells and propagated over 10 generations. SmaI digestions were performed at every even-numbered generation to evaluate the vectors' ability to retain ITR sequences. GenScript’s AAV-applied vectors demonstrated significantly superior stability of ITR sequences during plasmid propagation.
Plasmids containing three GOI sequences were constructed using four vector backbones. Maxiprep was performed from 100 mL cultures, and the final products were quantified. GenScript’s AAV applied vectors demonstrated a significant increase in plasmid yield compared to conventional vectors.
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