GenScript siRNA Target Finder
Use this tool to identify unique candidate siRNA target sequence within cDNA sequences.
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Instructions on using this siRNA design tool:
- Learn about our siRNA design strategy.
- Specify the length, GC%, range, and sequence region for each siRNA target site. Alternatively, you may use the default settings.
- Specify the target mRNA nucleotide sequence by accession number or gene ID. Alternatively, you may copy/paste the cDNA sequence into the appropriate box below.
- Specify the organism in which the RNA interference experiment is to be conducted. This is used to filter out non-unique sequences via BLAST/SmithWaterman search.
- Check the box at the bottom to design multiple RT-PCR primer sets at the same time.
- Click "Start." Our software will find siRNA target sites based on your specifications. The candidate sequences will be filtered to remove non-unique sequences by BLAST/SmithWaterman against a unique and comprehensive EST/mRNA collection. The BLAST/SmithWaterman search may take some time, so please be patient. The ranking of siRNA candidates is based on a proprietary algorithm using ΔE parameters.
