Tagged Protein Purification Resins

To simplify the purification of recombinant proteins by affinity chromatography, a tag of protein/peptide or other molecule with known size is usually incorporated into the proteins. The two most commonly used tags are 6×Histidine and Glutathione S-transferase (GST). Biotin and Streptavidin are also frequently attached to proteins of interest to enable simple affinity purification. GenScript provides several affinity resins for convenient and reliable purification of tagged proteins.

Application Product Technology Binding Capacity Vol. Cat. No.
Purification of His-tagged proteins purification High Affinity Ni-Charged Resin His-tagged protein capture through pre-charged nickel ion under native or denaturing conditions >20 mg 6xHis-tagged protein/ml (CV) 10 ml
25 ml
500 ml
Ni Resin FF >40mg 6xHis-tagged protein/ml (CV). 5 ml L00465-5
25 ml L00465-25
Purification of GST fusion proteins and others expressed in E. coli, insect cells and mammalian cells. Glutathione Resin GST fusion protein capture through reduced glutathione >20 mg GST-fusion proteins (26 kDa)/ml medium 10 ml
Purification of biotinylated molecules Streptavidin Resin Biotinylated molecule capture through highly pre-coupled streptavidin >120 nmol of D-biotin per ml of column-volume 5 ml
Purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. Anti-DYKDDDDK G1 Affinity Resin The DYKDDDDK-tagged protein capture through pre-coupled DYKDDDDK mAbs Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin 1 ml
5 ml
10 ml
25 ml

Ordering Information

Cat. No.
Product Name
L00223-25 High Affinity Ni-Charged Resin
25 ml
L00223-10 High Affinity Ni-Charged Resin
10 ml
L00206 Glutathione Resin
10 ml
L00207 GST Fusion Protein Purification Kit
1 kit
L00208 Protein Expression and Purification Kit
1 kit
L00353 Streptavidin Resin
5 ml

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