Overview

To simplify the purification of recombinant proteins by affinity chromatography, a tag of protein/peptide or other molecule with known size is usually incorporated into the proteins. The two most commonly used tags are 6×Histidine and Glutathione S-transferase (GST). Biotin and Streptavidin are also frequently attached to proteins of interest to enable simple affinity purification. GenScript provides several affinity resins for convenient and reliable purification of tagged proteins.

Application Product Ligand used Binding capacity Size Cat.No.
His-tagged protein purification High Affinity Ni-charged Resin Ni-NTA-charged resin 45-60 mg 6xHis-tagged protein (27 kD) /ml settled resin 10 ml
25 ml
500 ml
L00223
Ni Resin FF Ni-IDA-charged FPLC-compatible resin >40 mg 6xhistidine-tagged protein /ml settled resin 5 ml
25 ml
L00465
High Affinity Ni-Charged Resin FF Ni-NTA-charged FPLC-compatible resin >45 mg of histidine-tagged protein /ml settled resin 5/25/100 ml L00666
His-tagged protein purification (Prepacked Column) High Affinity Ni-Charged Resin FF Prepacked Column Ni-NTA-charged FPLC-compatible resin in a prepacked column >45 mg of histidine-tagged protein /ml settled resin 2 /5 /25 ml L00683
Ni-IDA Resin FF Prepacked Column Ni-IDA-charged FPLC-compatible resin in a prepacked column >40 mg of histidine-tagged protein /ml settled resin 2 /5 /25 ml L00684
GST fusion protein purification Glutathione Resin Glutathione 35-45 mg GST-tagged protein (26 kD) /ml settled resin  10 ml L00206
GST Fusion Protein Purification Kit 1 kit L00207
Purification of DYKDDDDK tagged protein MonoRab™ Anti-DYKDDDDK Affinity Resin MonoRab™ Anti-DYKDDDDK antibody Approximately 1.5 mg DYKDDDDK-tagged protein/ml settled resin 1 ml
5 ml
25 ml
L00766
Anti-DYKDDDDK G1 Affinity Resin Anti-DYKDDDDK G1 antibody Approximately 1 mg DYKDDDDK-tagged protein/ml settled resin 1 ml
5 ml
10 ml
25 ml
L00432
Purification of biotinylated protein, antibody, lectin etc. Streptavidin Resin Streptavidin 120 nmol D-Binotin/ml settled resin 5 ml L00353
Immobilization of sulfhydryl-containing peptides, proteins and other ligands for following purification High-Affinity Iodoacetyl Resin   >9 mg Goat IgG/ml settled resin 10 ml L00403
1 kit L00404
High-Affinity Antibody Purification Kit
Immunoprecipitation Anti-His Affinity Resin THE™ anti-His monoclonal antibody >0.8 mg of histidine-tagged protein /ml settled resin 1/5 ml L00439
Chicken IgY Precipitating Resin Goat Anti-Chicken IgY Immunoprecipitation of chicken IgY or the enrichment of chicken primary IgY‐protein complexes 1 ml L00405
Anti-DYKDDDDK IP Resin Mouse anti-DYKDDDDK monoclonal antibody Immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex 1 ml L00425

Ordering Information

Cat. No. Product Name Quantity Price Order
L00207
GST Fusion Protein Purification Kit
$139.00 1 kit
 
L00223
High Affinity Ni-Charged Resin
$80.00
 
L00353
Streptavidin Resin
$152.90 5 ml Resin (Total 10 ml)
 
L00432
Anti-DYKDDDDK G1 Affinity Resin
$281.00
 
L00465
Ni Resin FF
$49.00
 
L00666
High Affinity Ni-Charged Resin FF
$62.00
 
L00683
High Affinity Ni-Charged Resin FF Prepacked Column
$65.00
 
L00684
Ni-IDA Resin FF Prepacked Column
$45.00
 

Resins for Immunoprecipitation

Cat. No. Product Name Quantity Price Order
L00425
Anti-DYKDDDDK IP Resin
$199.00 1 ml Resin (Total 2 ml)
 
L00439
Anti-His Affinity Resin
$399.00
 

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