Mastering the IHC Workflow: Key Insights for Successful Antibody Development with GenScript

Immunohistochemistry (IHC) is a vital technique that uses antibodies to locate antigens in tissues, with applications in cancer research and diagnostics. However, IHC's multistep workflow demands careful optimization, particularly in antibody-antigen binding. To achieve reliable results, it's crucial to follow mindful antibody development practices. This article explores the essentials of the IHC workflow and provides four key considerations for successful IHC antibody development with GenScript. When screening antibodies, using an overexpression cell line and positive control antibodies for IHC-cell-based ELISA or Flow cytometry ensures consistent results. If no such cell line is available, Indirect ELISA is a suitable alternative for antibody screening.

Immunohistochemistry (IHC) is a vital method for precise antigen localization within tissues using monoclonal and polyclonal antibodies. While it plays a pivotal role in various research fields and molecular diagnostics, its challenging multistep workflow, encompassing stages like tissue processing and antigen retrieval, requires meticulous optimization. The specificity of antibody-antigen binding is fundamental, and this article explores key aspects of the IHC workflow and four crucial considerations for successful IHC antibody development with GenScript.

This application note highlights: