CRISPR Based T Cell Editing: Large knock-ins in human T cells using non-viral HDR templates

Current efforts at reprogramming T cells for therapeutic purposes rely on using recombinant viral vectors. Unfortunately, viral vectors do not target transgenes to specific genomic sites. Moreover, the manufacturing and testing of effective viral vectors is often a lengthy and expensive process, which slows research progress and clinical use. However, recent studies have shown that re-engineering T cells in a specific and efficient manner is possible using homology-directed repair (HDR).

In this webinar, we will cover

The advantages of using HDR versus recombinant viral vectors when modifying T cell genomes.

How long double-stranded and single-stranded DNA can serve as a non-viral HDR template.

A novel method that allows for the insertion of large DNA sequences (>1Kb) without a virus!

In this webinar, you will learn

Webinar Details

  • Date: Nov. 1st, 2018
  • Time: 11:00 - 12:00 PM EST
  • Speaker:
Theo Roth, Marson Lab, UCSF Theo Roth

Marson Lab, UCSF

Theodore Roth, MD/PhD student, Marson Lab, UCSF. Graduated from Stanford with a BS in Biology and an MS in Biomedical Informatics. Currently, under MD and PhD training at UCSF, with interest in Immunology, Genetics, Informatics, and Genome Editing in the context of Cellular Therapeutics.

Reprogramming human T cell function and specificity with non-viral genome targeting
Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel J, Pellerin L, Carmody D, Alkorta-Aranburu G, del Gaudio D, Matsumoto H, Morell M, Mao Y, Cho M, Quadros RM, Gurumurthy CB, Smith B, Haugwitz M, Hughes SH, Weissman JS, Schumann K, Esensten JH, May AP, Ashworth A, Kupfer GM, Greeley SAW, Bacchetta R, Meffre E, Roncarolo MG, Romberg N, Herold KC, Ribas A, Leonetti MD, Marson A. Nature. 2018 Jul;559(7714):405-409.

CRISPR RNP User Manual
CRISPR RNP User Manual

A guide on how to use CRISPR RNP for targeted genome editing.

Free Download

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